| Explant outgrowth, propagation and characterization of human pericytes. | |
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MedLine Citation:
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PMID: 20618694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Pericytes are critical cellular components of the microvasculature that play a major role in vascular development and pathologies, yet their study has been hindered by lack of a standardized method for their isolation and growth. Here we report a method for culturing human pericytes from a readily available tissue source, placenta, and provide a thorough characterization of resultant cell populations. METHODS: We developed an optimized protocol for obtaining pericytes by outgrowth from microvessel fragments recovered after enzymatic digestion of human placental tissue. We characterized outgrowth populations by immunostaining, by gene expression analysis, and by functional evaluation of cells implanted in vivo. RESULTS: Our approach yields human pericytes that may be serially expanded in culture and that uniformly express the cellular markers NG2, CD90, CD146, alpha-SMA, and PDGFR-beta, but lack markers of smooth muscle cells, endothelial cells, and leukocytes. When co-implanted with human endothelial cells into C.B-17 SCID/bg mice, human pericytes invest and stabilize developing human endothelial cell-lined microvessels. CONCLUSIONS: We conclude that our method for culturing pericytes from human placenta results in the expansion of functional pericytes that may be used to study a variety of questions related to vascular biology. |
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Authors:
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Cheryl L Maier; Benjamin R Shepherd; Tai Yi; Jordan S Pober |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Microcirculation (New York, N.Y. : 1994) Volume: 17 ISSN: 1549-8719 ISO Abbreviation: Microcirculation Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-29 Revised Date: 2012-03-15 |
Medline Journal Info:
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Nlm Unique ID: 9434935 Medline TA: Microcirculation Country: United States |
Other Details:
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Languages: eng Pagination: 367-80 Citation Subset: IM |
Affiliation:
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Departments of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Capillary Permeability Cell Culture Techniques / methods* Cell Separation Endothelial Cells / transplantation Female Gene Expression Profiling Humans Immunophenotyping Mice Mice, SCID Microvessels / cytology Myocytes, Smooth Muscle / cytology Oligonucleotide Array Sequence Analysis Pericytes / cytology*, physiology*, transplantation Placenta / blood supply, cytology Pregnancy Transplantation, Heterologous |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL051014-17/HL/NHLBI NIH HHS; R01 HL085416-05/HL/NHLBI NIH HHS; R01 HL085416-07/HL/NHLBI NIH HHS; R01-HL051014/HL/NHLBI NIH HHS; R01-HL085416/HL/NHLBI NIH HHS; T32-GM-007205/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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