| Experiments in cardiac xenotransplantation. Response to intrathymic xenogeneic cells and intravenous cobra venom factor. | |
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MedLine Citation:
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PMID: 8412256 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Permanent tolerance to an experimental cardiac allograft can be achieved by pretransplantation intrathymic inoculation of donor-specific lymphoid cells. We studied the effects of intrathymic inoculation of xenogeneic cells and intravenous cobra venom factor in a rodent model of cardiac xenotransplantation. Lewis rats underwent intraabdominal heterotopic heart transplantation with Syrian hamster donors. In untreated animals, mean graft survival time was 3 days. Five rats had 1 ml of antilymphocyte serum administered intraperitoneally. One day later, 2.5 x 10(7) hamster spleen cells were inoculated into the thymus under direct vision. Twenty-one days after antilymphocyte serum was given, heterotopic heart transplantation with a hamster donor was carried out. In all cases, rejection was accelerated and occurred between 20 minutes and 1 day after transplantation. Mean graft survival time was 5.2 hours (p < 0.0001 versus control). Six animals treated with antilymphocyte serum and intrathymic xenogeneic cells had 0.5 ml of cobra venom factor, a complement antagonist, administered intravenously 3 hours before transplantation and every other day thereafter. Mean graft survival was 3 days, which was not different from the response of naive animals. Animals treated with antilymphocyte serum only had no prolongation of graft survival (mean survival time 3 days, p = not significant). Animals treated with cobra venom factor alone (n = 5) before transplantation and on alternate days subsequently had mild graft prolongation with a mean survival time of 4 days (p = 0.0133). In contrast to experimental allograft models, intrathymic inoculation of xenogeneic cells produces hyperacute rejection in these naturally concordant species. The administration of cobra venom factor abrogates the hyperacute response, but the combination of cobra venom factor and intrathymic inoculation does not produce long-term graft survival. |
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Authors:
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M Mohiuddin; G Kline; Z Shen; V Ruggiero; S Rostami; V J DiSesa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of thoracic and cardiovascular surgery Volume: 106 ISSN: 0022-5223 ISO Abbreviation: J. Thorac. Cardiovasc. Surg. Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1993-11-17 Completed Date: 1993-11-17 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0376343 Medline TA: J Thorac Cardiovasc Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 632-5 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, Hospital of the University of Pennsylvania, Philadelphia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cobra Venoms / immunology, therapeutic use* Cricetinae Graft Rejection / immunology Graft Survival / immunology* Heart Transplantation / methods* Lymphocyte Transfusion* / methods Male Mesocricetus Rats Rats, Inbred Lew Spleen / cytology, immunology Thymus Gland / immunology Transplantation, Heterologous* |
| Chemical | |
Reg. No./Substance:
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0/Cobra Venoms; 0/cobra venom factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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