Document Detail


Experimental study on repair of the facial nerve with Schwann cells transfected with GDNF genes and PLGA conduits.
MedLine Citation:
PMID:  18607939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONCLUSIONS: Schwann cells transfected by GDNF genes + PLGA were superior to Schwann cells + PLGA and direct anastomesis. This is a new and effective strategy for repair of facial nerve defects. OBJECTIVE: To evaluate the effect of bioactive artificial nerve conduits in the repair of facial nerve defects in Sprague-Dawley rats. MATERIALS AND METHODS: Schwann cells were harvested and transfected with PcDNA3.1 (+)/GDNF. After injection with Schwann cells, the conduits were cultured in the culture medium for 2 weeks. Thirty female Sprague-Dawley rats were selected and randomly divided into three groups (A, B, and C), which were treated as follows: A, direct anastomesis; B, Schwann cells + PLGA conduits; C, Schwann cells transfected by GDNF genes + PLGA conduits. General observation, electrophysiological study, histological study, and image analysis were performed 2 weeks, 1 month, 2 months, and 3 months postoperatively. RESULTS: The recovery of nerve regeneration and electrophysiological results in group C were superior to those in groups A and B; the difference was statistically significant (p < 0.01).
Authors:
Liang Zhou; Huai-Dong Du; Hong-Bin Tian; Cai Li; Jie Tian; Jack J Jiang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  128     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2009-02-25     Completed Date:  2009-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  1266-72     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Fudan University Affiliated Eye, Ear, Nose and Throat Hospital, Shanghai, China. zhlwc@online.sh.cn
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MeSH Terms
Descriptor/Qualifier:
Action Potentials
Animals
Cells, Cultured
Facial Nerve / cytology,  physiology*
Facial Nerve Injuries / therapy*
Female
Glial Cell Line-Derived Neurotrophic Factor / analysis,  genetics*
Guided Tissue Regeneration*
Lactic Acid*
Male
Nerve Regeneration*
Polyglycolic Acid*
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Schwann Cells* / cytology
Tissue Engineering*
Transfection*
Chemical
Reg. No./Substance:
0/Glial Cell Line-Derived Neurotrophic Factor; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

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