Document Detail

Experimental study on repair of the facial nerve with Schwann cells transfected with GDNF genes and PLGA conduits.
MedLine Citation:
PMID:  18607939     Owner:  NLM     Status:  MEDLINE    
CONCLUSIONS: Schwann cells transfected by GDNF genes + PLGA were superior to Schwann cells + PLGA and direct anastomesis. This is a new and effective strategy for repair of facial nerve defects. OBJECTIVE: To evaluate the effect of bioactive artificial nerve conduits in the repair of facial nerve defects in Sprague-Dawley rats. MATERIALS AND METHODS: Schwann cells were harvested and transfected with PcDNA3.1 (+)/GDNF. After injection with Schwann cells, the conduits were cultured in the culture medium for 2 weeks. Thirty female Sprague-Dawley rats were selected and randomly divided into three groups (A, B, and C), which were treated as follows: A, direct anastomesis; B, Schwann cells + PLGA conduits; C, Schwann cells transfected by GDNF genes + PLGA conduits. General observation, electrophysiological study, histological study, and image analysis were performed 2 weeks, 1 month, 2 months, and 3 months postoperatively. RESULTS: The recovery of nerve regeneration and electrophysiological results in group C were superior to those in groups A and B; the difference was statistically significant (p < 0.01).
Liang Zhou; Huai-Dong Du; Hong-Bin Tian; Cai Li; Jie Tian; Jack J Jiang
Related Documents :
14609669 - Stabilisation of cables of fibronectin with micromolar concentrations of copper: in vit...
16932869 - Specific response of osteoblast-like cells on hydroxyapatite layer containing serum pro...
9449069 - Embryonic schwann cell development: the biology of schwann cell precursors and early sc...
16180049 - Establishment of immortalized schwann cells from sandhoff mice and corrective effect of...
19674089 - Gaba release from cerebellar stellate cells is developmentally regulated by presynaptic...
15797019 - Erbb3 and erbb2 are essential for schwann cell migration and myelination in zebrafish.
11536739 - Plant cell growth responds to external forces and the response requires intact microtub...
25457229 - Modeling intrinsic heterogeneity and growth of cancer cells.
15453639 - A comparison of the effects of ocular preservatives on mammalian and microbial atp and ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  128     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2009-02-25     Completed Date:  2009-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  1266-72     Citation Subset:  IM    
Department of Otolaryngology-Head and Neck Surgery, Fudan University Affiliated Eye, Ear, Nose and Throat Hospital, Shanghai, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Action Potentials
Cells, Cultured
Facial Nerve / cytology,  physiology*
Facial Nerve Injuries / therapy*
Glial Cell Line-Derived Neurotrophic Factor / analysis,  genetics*
Guided Tissue Regeneration*
Lactic Acid*
Nerve Regeneration*
Polyglycolic Acid*
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Schwann Cells* / cytology
Tissue Engineering*
Reg. No./Substance:
0/Glial Cell Line-Derived Neurotrophic Factor; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Activation of vocal fold healing with topical vitamin A in rabbits.
Next Document:  Facial nerve course in congenital aural atresia--identified by preoperative CT scanning and surgical...