| Experimental study on repair of the facial nerve with Schwann cells transfected with GDNF genes and PLGA conduits. | |
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MedLine Citation:
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PMID: 18607939 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CONCLUSIONS: Schwann cells transfected by GDNF genes + PLGA were superior to Schwann cells + PLGA and direct anastomesis. This is a new and effective strategy for repair of facial nerve defects. OBJECTIVE: To evaluate the effect of bioactive artificial nerve conduits in the repair of facial nerve defects in Sprague-Dawley rats. MATERIALS AND METHODS: Schwann cells were harvested and transfected with PcDNA3.1 (+)/GDNF. After injection with Schwann cells, the conduits were cultured in the culture medium for 2 weeks. Thirty female Sprague-Dawley rats were selected and randomly divided into three groups (A, B, and C), which were treated as follows: A, direct anastomesis; B, Schwann cells + PLGA conduits; C, Schwann cells transfected by GDNF genes + PLGA conduits. General observation, electrophysiological study, histological study, and image analysis were performed 2 weeks, 1 month, 2 months, and 3 months postoperatively. RESULTS: The recovery of nerve regeneration and electrophysiological results in group C were superior to those in groups A and B; the difference was statistically significant (p < 0.01). |
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Authors:
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Liang Zhou; Huai-Dong Du; Hong-Bin Tian; Cai Li; Jie Tian; Jack J Jiang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta oto-laryngologica Volume: 128 ISSN: 0001-6489 ISO Abbreviation: Acta Otolaryngol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2009-02-25 Completed Date: 2009-03-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370354 Medline TA: Acta Otolaryngol Country: Norway |
Other Details:
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Languages: eng Pagination: 1266-72 Citation Subset: IM |
Affiliation:
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Department of Otolaryngology-Head and Neck Surgery, Fudan University Affiliated Eye, Ear, Nose and Throat Hospital, Shanghai, China. zhlwc@online.sh.cn |
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials Animals Cells, Cultured Facial Nerve / cytology, physiology* Facial Nerve Injuries / therapy* Female Glial Cell Line-Derived Neurotrophic Factor / analysis, genetics* Guided Tissue Regeneration* Lactic Acid* Male Nerve Regeneration* Polyglycolic Acid* Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Schwann Cells* / cytology Tissue Engineering* Transfection* |
| Chemical | |
Reg. No./Substance:
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0/Glial Cell Line-Derived Neurotrophic Factor; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid |
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