Document Detail

[Experimental study on protective effect of early motion on articular cartilage after joint allograft].
MedLine Citation:
PMID:  22332513     Owner:  NLM     Status:  In-Process    
OBJECTIVE: To investigate the protective effect of early motion on articular cartilage after joint allograft by performing a controlled trial between different post-operation strategies after joint allograft in an animal model.
METHODS: Twenty hemi-knee joints were harvested from 10 6-month-old New Zealand white rabbits (male or female, weighing 2.5-3.0 kg); 10 hemi-knee joints by deep frozen treatment (donors) were transplanted to unilateral knee joints (recipients) of 10 6-month-old Chinchilla rabbits (male or female, weighing 2.5-3.0 kg), which were divided into early motion group (n = 5) and sustained fixation group (n = 5); and 10 hemi-knee joints were used as blank control (n = 5) and frozen control (n = 5). The articular cartilage of allogenic joints was detected by X-ray film, gross, and histology at 6 weeks after operation.
RESULTS: Gross observation: no obvious limitation of joint movements was observed in early motion group, but obvious limitation in sustained fixation group. X-ray films: the bone ends between donor and recipient healed well with good paraposition and alignment on the operation day and 2 weeks after operation; at 6 weeks, angulation deformity was observed in early motion group of 3 rabbits, and paraposition and alignment were satisfactory in sustained fixation group. Histological observation: HE staining showed that the chondrocytes had normal quantity and morphology with few nuclear fragmentation and karyolysis in early motion group, but the quantity of chondrocytes sharply decreased with dissolved nuclei and numerous fibrous tissues in the cartilage matrix in sustained fixation group. The cell survival rate of the early motion group (49.66% +/- 2.15%) was significantly higher than that of the sustained fixation group (20.68% +/- 1.24%) (P < 0.05). Scanning electron microscopy observation: nuclear membrane was intact with chromatin condensation and edema of mitochondria and rough surfaced endoplasmic reticulum in early motion group, and that the membrane of chondrocyte vanished with blurring border between chondrocyte and matrix, rupture of nuclear membrane and the disappearance of chromatin and organelles could be found in sustained fixation group.
CONCLUSION: Early motion has protective effect on articular cartilage after joint allograft, but cannot completely prevent degeneration of the allogenic articular cartilage.
Xiang Li; Youle Zhang; Jiuzheng Deng
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery     Volume:  26     ISSN:  1002-1892     ISO Abbreviation:  Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-02-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9425194     Medline TA:  Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi     Country:  China    
Other Details:
Languages:  chi     Pagination:  26-30     Citation Subset:  IM    
Department of Hand Surgery, Beijing Jishuitan Hospital, the Fourth Clinical School Medicine of Peking University, Beijing, 100035, P R China.
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