Document Detail


Experimental study of cryopreserved allogeneic transfer of vessel: preliminary report.
MedLine Citation:
PMID:  8133771     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has long been known that the antigenicity of tissues is depressed when they are kept at a very low temperature. In this study, Lewis rats and Brown Norway (B.N.) rats were used because these species are significantly dissimilar at the major histocompatibility complex (MHC) Femoral arteries were harvested from Lewis rats, frozen while lowering the temperature in a stepwise fashion to -70 degrees C. After three weeks of cryogenic storage, the arteries were transplanted into Lewis and B.N. rats. At various times from the first to the twelfth week after surgery, grafted vessels were observed, and pathological and electron microscopic studies were carried out. The vessels were found to be in good condition at the end of the 12 week observation period. These results strongly suggest that prolonged cryopreservation of vessels makes allotransplantation without immunosuppression possible.
Authors:
M Takeishi; Y Hirase; T Kojima
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microsurgery     Volume:  15     ISSN:  0738-1085     ISO Abbreviation:  Microsurgery     Publication Date:  1994  
Date Detail:
Created Date:  1994-04-20     Completed Date:  1994-04-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8309230     Medline TA:  Microsurgery     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  55-62     Citation Subset:  IM    
Affiliation:
Department of Plastic and Reconstructive Surgery, Jikei University School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arteries / immunology,  transplantation*,  ultrastructure
Cryopreservation*
Immunosuppression
Male
Rats
Rats, Inbred BN
Rats, Inbred Lew
Tissue Preservation / methods*
Transplantation, Homologous
Vascular Patency

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