Document Detail


Experimental streptozotocin-reduced diabetes and intestinal glucose metabolism in the rat, in vivo and in vitro.
MedLine Citation:
PMID:  8590788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intestine has a high glycolytic activity, but its metabolic role could be altered in diabetes mellitus. The aim of the present work was to investigate in vivo the glucose retained and the lactate produced by the intestine of normal and diabetic rats and in vitro the effect of different arterial glucose concentrations on glucose utilization and lactate, alanine, and pyruvate production in normal and diabetic rats when the glucose is supplied to the intestine exclusively via the vascular route. In vivo, the normal and diabetic rats retained similar percentages of the arterially supplied glucose (14.7 +/- 2.4, respectively). In vitro, when the preparations were perfused under hyperglycemic conditions, the glucose consumed, as a fraction of the quantity infused, was significantly lower (P < 0.05) in the diabetic (247.0 +/- 22.8 mumol/mmol infused glucose) than in normal (315 +/- 16.3 mumol/mmol infused glucose rats) rats. The lactate produced was significantly higher in diabetic than in normal rats whether the preparations were perfused under isoglycemic (P < 0.01; 1916.4 +/- 124.0 vs vs 1284 +/- 67.7 mumol/mmol consumed glucose) or hyperglycemic (P < 0.05; 1356.4 +/- 199.7 vs 898.0 +/- 87.3 mumol/mmol consumed glucose) conditions. There was significantly (P < 0.05) greater alanine release from the diabetic (123.7 +/- 21.8 mumol/mmol consumed glucose) than from the normal (40.7 +/- 10.3 mumol/mmol consumed glucose) rat preparations perfused under isoglycemic conditions.
Authors:
M A Tormo; M A Gómez-Zubeldia; F Ropero; M Muñoz-Casillas; J C Moreno; J E Campillo
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Acta diabetologica     Volume:  32     ISSN:  0940-5429     ISO Abbreviation:  Acta Diabetol     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-04-01     Completed Date:  1996-04-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9200299     Medline TA:  Acta Diabetol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  182-6     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Medicine, University of Extremadura, Badajoz, Spain.
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MeSH Terms
Descriptor/Qualifier:
Alanine / metabolism
Animals
Blood Glucose / metabolism
Diabetes Mellitus, Experimental / metabolism*
Glucose / metabolism*
Glycolysis
Intestine, Small / metabolism*
Lactates / metabolism
Lactic Acid
Male
Osmolar Concentration
Pyruvates / metabolism
Pyruvic Acid
Rats
Rats, Wistar
Reference Values
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 50-99-7/Glucose; 56-41-7/Alanine

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