| Experimental spinal cord injury induced an increase of extracellular ascorbic acid concentration in anesthetized rats: a microdialysis study. | |
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MedLine Citation:
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PMID: 16033694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ascorbic acid plays important roles in mammalian central nervous system. We employed an on-line analytical system to monitor the extracellular ascorbic acid concentrations in anesthetized rat spinal cord before and after the experimental injury. A microdialysis probe (216 microm od, 200 microm id, 3 mm in length) was implanted into an anesthetized rat spinal cord (Thoratic-12). Microdialysis perfusate (2 microl/min) was collected in the sample loop (20 microl) of an on-line injector for direct injection onto a High Performance Liquid Chromatography (HPLC) system equipped with an electrochemical detector. Normal ascorbic acid concentrations in the spinal cord extracellular fluids ranged from 1.8 microM to 10.8 microM (mean +/- S.D. 5.6 +/- 2.4 microM, n = 8). The experimental spinal cord injury, induced by a lesion at T-10, gradually yet significantly increased the extracellular ascorbic acid levels. The effect of exogenous glutamate perfusion (0.2 mM, 2 mM, and 20 mM) through the microdialysis probe also increased the extracellular ascorbic acid concentrations in a dose dependent manner. These results suggested that the injury-induced ascorbic acid accumulation may result from elevated extracellular glutamate levels that are commonly observed in spinal cord injury. This on-line, continuous and automatic monitoring system can be applied to future investigations on the roles of ascorbic acid in spinal cord injuries. |
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Authors:
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Pi-Ju Tsai; Wen-Ying Chen; Shun-Fen Tzeng; Wei-Ming Liang; Chung-Shi Yang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-07-19 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 362 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-11-08 Completed Date: 2006-01-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 94-100 Citation Subset: IM |
Affiliation:
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Department of Education and Research, Taichung Veterans General Hospital, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anesthesia Animals Ascorbic Acid / metabolism* Extracellular Space / drug effects, metabolism* Glutamic Acid / pharmacology Male Microdialysis Rats Rats, Sprague-Dawley Spinal Cord Injuries / metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
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50-81-7/Ascorbic Acid; 56-86-0/Glutamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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