Document Detail

Experimental spinal cord injury induced an increase of extracellular ascorbic acid concentration in anesthetized rats: a microdialysis study.
MedLine Citation:
PMID:  16033694     Owner:  NLM     Status:  MEDLINE    
Ascorbic acid plays important roles in mammalian central nervous system. We employed an on-line analytical system to monitor the extracellular ascorbic acid concentrations in anesthetized rat spinal cord before and after the experimental injury. A microdialysis probe (216 microm od, 200 microm id, 3 mm in length) was implanted into an anesthetized rat spinal cord (Thoratic-12). Microdialysis perfusate (2 microl/min) was collected in the sample loop (20 microl) of an on-line injector for direct injection onto a High Performance Liquid Chromatography (HPLC) system equipped with an electrochemical detector. Normal ascorbic acid concentrations in the spinal cord extracellular fluids ranged from 1.8 microM to 10.8 microM (mean +/- S.D. 5.6 +/- 2.4 microM, n = 8). The experimental spinal cord injury, induced by a lesion at T-10, gradually yet significantly increased the extracellular ascorbic acid levels. The effect of exogenous glutamate perfusion (0.2 mM, 2 mM, and 20 mM) through the microdialysis probe also increased the extracellular ascorbic acid concentrations in a dose dependent manner. These results suggested that the injury-induced ascorbic acid accumulation may result from elevated extracellular glutamate levels that are commonly observed in spinal cord injury. This on-line, continuous and automatic monitoring system can be applied to future investigations on the roles of ascorbic acid in spinal cord injuries.
Pi-Ju Tsai; Wen-Ying Chen; Shun-Fen Tzeng; Wei-Ming Liang; Chung-Shi Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-07-19
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  362     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-08     Completed Date:  2006-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  94-100     Citation Subset:  IM    
Department of Education and Research, Taichung Veterans General Hospital, Taiwan.
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MeSH Terms
Ascorbic Acid / metabolism*
Extracellular Space / drug effects,  metabolism*
Glutamic Acid / pharmacology
Rats, Sprague-Dawley
Spinal Cord Injuries / metabolism*,  pathology
Reg. No./Substance:
50-81-7/Ascorbic Acid; 56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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