| Experimental protocol for activation-induced manganese-enhanced MRI (AIM-MRI) based on quantitative determination of Mn content in rat brain by fast T1 mapping. | |
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MedLine Citation:
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PMID: 19725135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In activation-induced manganese-enhanced MRI (AIM-MRI) experiments, differential accumulation of Mn in activated and silent brain areas is generally assessed using T(1)-weighted images and quantified by the enhancement of signal intensity (SI), calculated with reference to SI before Mn administration or to SI of brain regions unaffected by the specific stimulus. However, SI enhancement can be unreliable when animals are removed from and reinserted into the magnet. We have developed an experimental protocol based on repeated intraperitoneal (i.p.) injections of Mn, quantitative determination of T(1), and coregistration of images to a rat brain atlas that allows absolute quantification of Mn concentration in selected brain areas. Results showed that interanimal variability of postcontrast T(1) values was very low (compared to the experimental error in T(1) determinations) allowing detection of differential regional Mn uptake in stimulated and unstimulated animals. In addition we have determined in vivo relaxivity of Mn in brain tissue and its frequency dependence. |
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Authors:
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S Tambalo; A Daducci; S Fiorini; F Boschi; M Mariani; M Marinone; A Sbarbati; P Marzola |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 62 ISSN: 1522-2594 ISO Abbreviation: Magn Reson Med Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-05 Completed Date: 2010-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 1080-4 Citation Subset: IM |
Copyright Information:
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(c) 2009 Wiley-Liss, Inc. |
Affiliation:
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Department of Morphological and Biomedical Sciences, University of Verona, Verona, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Brain / anatomy & histology*, physiology* Brain Mapping / methods* Chlorides / diagnostic use*, pharmacokinetics* Computer Simulation Contrast Media / pharmacokinetics Image Enhancement / methods Image Interpretation, Computer-Assisted / methods* Magnetic Resonance Imaging / methods* Male Manganese Compounds / diagnostic use*, pharmacokinetics* Models, Neurological Rats Rats, Sprague-Dawley Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Contrast Media; 0/Manganese Compounds; 7773-01-5/manganese chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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