Document Detail

Experimental protocol for activation-induced manganese-enhanced MRI (AIM-MRI) based on quantitative determination of Mn content in rat brain by fast T1 mapping.
MedLine Citation:
PMID:  19725135     Owner:  NLM     Status:  MEDLINE    
In activation-induced manganese-enhanced MRI (AIM-MRI) experiments, differential accumulation of Mn in activated and silent brain areas is generally assessed using T(1)-weighted images and quantified by the enhancement of signal intensity (SI), calculated with reference to SI before Mn administration or to SI of brain regions unaffected by the specific stimulus. However, SI enhancement can be unreliable when animals are removed from and reinserted into the magnet. We have developed an experimental protocol based on repeated intraperitoneal (i.p.) injections of Mn, quantitative determination of T(1), and coregistration of images to a rat brain atlas that allows absolute quantification of Mn concentration in selected brain areas. Results showed that interanimal variability of postcontrast T(1) values was very low (compared to the experimental error in T(1) determinations) allowing detection of differential regional Mn uptake in stimulated and unstimulated animals. In addition we have determined in vivo relaxivity of Mn in brain tissue and its frequency dependence.
S Tambalo; A Daducci; S Fiorini; F Boschi; M Mariani; M Marinone; A Sbarbati; P Marzola
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  62     ISSN:  1522-2594     ISO Abbreviation:  Magn Reson Med     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2010-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1080-4     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
Department of Morphological and Biomedical Sciences, University of Verona, Verona, Italy.
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MeSH Terms
Brain / anatomy & histology*,  physiology*
Brain Mapping / methods*
Chlorides / diagnostic use*,  pharmacokinetics*
Computer Simulation
Contrast Media / pharmacokinetics
Image Enhancement / methods
Image Interpretation, Computer-Assisted / methods*
Magnetic Resonance Imaging / methods*
Manganese Compounds / diagnostic use*,  pharmacokinetics*
Models, Neurological
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Chlorides; 0/Contrast Media; 0/Manganese Compounds; 7773-01-5/manganese chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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