| Experimental ocular tuberculosis in guinea pigs. | |
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MedLine Citation:
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PMID: 19752425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye. METHODS: Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs received high-dose infection and were treated with the first-line anti-TB drugs isoniazid, rifampin, and pyrazinamide. Development of ocular TB lesions was documented by histological analysis, acid-fast staining, and real-time polymerase chain reaction for M tuberculosis DNA. RESULTS: Untreated guinea pigs developed pulmonary and extrapulmonary TB. Ocular TB, primarily involving the uvea, developed in 5 of 12 eyes (42%). Uveal granulomatous lesions showed the presence of acid-fast organisms and M tuberculosis DNA. In treated animals, none of the 8 eyes examined revealed the presence of acid-fast organisms; however, there was mild nongranulomatous uveitis in 4 eyes. CONCLUSIONS: Mycobacterium tuberculosis delivered via aerosol to guinea pigs results in extrapulmonary dissemination to the eye. Of significance, intraocular changes in this model include granulomatous inflammation and the presence of acid-fast organisms, as seen in human cases of ocular TB. Clinical Relevance The guinea pig model may provide greater insight into the pathogenesis of intraocular TB and assist in the development of novel modalities to treat this global infectious disease. |
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Authors:
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Narsing A Rao; Thomas A Albini; Mirnalini Kumaradas; Michael L Pinn; Mostafa M Fraig; Petros C Karakousis |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of ophthalmology Volume: 127 ISSN: 1538-3601 ISO Abbreviation: Arch. Ophthalmol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-15 Completed Date: 2009-09-28 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 7706534 Medline TA: Arch Ophthalmol Country: United States |
Other Details:
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Languages: eng Pagination: 1162-6 Citation Subset: AIM; IM |
Affiliation:
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Doheny Eye Institute and Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aerosols Animals Antitubercular Agents / therapeutic use DNA, Bacterial / analysis Disease Models, Animal* Guinea Pigs Isoniazid / therapeutic use Lung / microbiology, pathology Mycobacterium tuberculosis / genetics, isolation & purification, pathogenicity* Polymerase Chain Reaction Pyrazinamide / therapeutic use Rifampin / therapeutic use Spleen / microbiology Tuberculosis, Ocular / drug therapy, microbiology*, pathology Tuberculosis, Pulmonary / drug therapy, microbiology*, pathology |
| Grant Support | |
ID/Acronym/Agency:
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AI064229/AI/NIAID NIH HHS; EY 03040/EY/NEI NIH HHS; P30 EY003040-30/EY/NEI NIH HHS; R01 EY017347-03/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aerosols; 0/Antitubercular Agents; 0/DNA, Bacterial; 13292-46-1/Rifampin; 54-85-3/Isoniazid; 98-96-4/Pyrazinamide |
| Comments/Corrections | |
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