| Experimental and natural borna disease virus infections: presence of viral RNA in cells of the peripheral blood. | |
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MedLine Citation:
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PMID: 10973698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells of the peripheral blood of experimentally and naturally borna disease virus (BDV)-infected animals and of human psychiatric patients and healthy individuals were analyzed for the presence of viral RNA using a BDV-p40-specific nested reverse transcription-polymerase chain reaction (RT-PCR). The assay proved to be highly sensitive as 10 RNA molecules were reproducibly amplified. BDV RNA was detected in blood cells of experimentally infected immunocompetent mice and rats. Mice were persistently infected without showing clinical signs of borna disease (BD), whereas the rats suffered from acute BD. Among 19 horses examined, five were positive for viral RNA in the blood. In a flock of sheep with a history of BD, 1 out of 25 clinically healthy animals was positive. BDV RNA was also detected in cells of the peripheral blood of 10 out of 27 selected humans with psychiatric disorders, and in 2 out of 13 healthy individuals. Remarkably, BDV-specific RNA was present in some cases in the absence of BDV-specific antibodies. Sequence analysis of PCR products confirmed the specificity of the amplification system. The presence of BDV RNA in the blood of naturally and experimentally BDV-infected individuals may point to an incidental but relevant role of blood for the spread of BDV in the infected organism, as well as for the transmission of BDV to other individuals. |
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Authors:
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T W Vahlenkamp; H K Enbergs; H Müller |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Veterinary microbiology Volume: 76 ISSN: 0378-1135 ISO Abbreviation: Vet. Microbiol. Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-11-22 Completed Date: 2000-11-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7705469 Medline TA: Vet Microbiol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 229-44 Citation Subset: IM |
Affiliation:
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Faculty of Veterinary Medicine, Institute of Virology, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany. virology@vetmed.uni-leipzig.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Borna Disease / genetics, virology* Borna disease virus / isolation & purification* Humans Injections, Intraventricular Leukocytes Leukocytes, Mononuclear / virology* Mice Mice, Inbred Strains Molecular Sequence Data RNA, Viral / blood* Rats Rats, Inbred Lew Reverse Transcriptase Polymerase Chain Reaction / veterinary Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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