Document Detail

Experimental models for studying the effects induced by staphylococcal toxins A and B on human keratinocytes in culture
MedLine Citation:
PMID:  1817457     Owner:  NLM     Status:  MEDLINE    
The toxic effects of the two serotypes of staphylococcal exotoxin: exfoliatin A (ETA) and B (ETB) on two experimental models: organotypic cultures of the skin and cellular cultures of the epidermis reconstructed "in vitro" have been studied. The results show that, in both cases, purified ETB (Fig. 4a, 6-6a, b, c) reproduces the characteristics of Lyell's Staphylococcal Syndrome that is the intraepidermal cleavage either between the granulosa and the spinous layers or at the granulosa layer of the epidermis. As the images of the LM and EM demonstrate, purified ETA behaves differently in the two experimental models; in fact, it produces the same effect as purified toxin B on the organotypic cultures (Fig. 3a, b), whereas it causes no alterations in the epithelial cultures reproduced "in vitro" (Fig. 5a, b).
A Arco; M Faure; Y Piemont; J Thivolet
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Archivio italiano di anatomia e di embriologia. Italian journal of anatomy and embryology     Volume:  96     ISSN:  0004-0223     ISO Abbreviation:  Arch Ital Anat Embriol     Publication Date:    1991 Jul-Sep
Date Detail:
Created Date:  1992-07-09     Completed Date:  1992-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985162R     Medline TA:  Arch Ital Anat Embriol     Country:  ITALY    
Other Details:
Languages:  ita     Pagination:  185-99     Citation Subset:  IM    
Dipartimento di Biomorfologia, Università di Messina.
Vernacular Title:
Modelli sperimentali per lo studio degli effetti indotti dalla tossina staphylococcica A. e B. su cheratinociti umani in coltura.
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MeSH Terms
Bacterial Toxins / pharmacology*
Cells, Cultured
Epidermal Necrolysis, Toxic / pathology
Hemolysin Proteins*
Keratinocytes / drug effects*,  ultrastructure
Microscopy, Electron
Sphingomyelin Phosphodiesterase*
Reg. No./Substance:
0/Bacterial Toxins; 0/Hemolysin Proteins; 0/staphylococcal alpha-toxin; EC Phosphodiesterase; EC protein, Staphylococcus aureus

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