| Experimental methods for scanning unnatural amino Acid mutagenesis. | |
| | |
MedLine Citation:
|
PMID: 21956563 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Site-specific incorporation of unnatural amino acids into proteins in vivo relies on the genetic reassignment of nonsense or quadruplet codons. Here, we describe a general procedure for the random introduction of these codons into open reading frames resulting in protein libraries that are scanned with unnatural amino acid residues. These libraries can enable large-scale mutagenesis experiments aimed at understanding and improving protein function. |
| | |
Authors:
|
Jia Liu; T Ashton Cropp |
Related Documents
:
|
16917403 - Postprandial stomach contents have multiple acid layers. 17534523 - Sugar sensing based on induced ph changes. 8306943 - The effect of digitonin and altered thyroid status on palmitic acid oxidation by isolat... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 794 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
|
Created Date: 2011-09-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 187-97 Citation Subset: IM |
Affiliation:
|
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Use of Unnatural Amino Acids to Probe Structure-Activity Relationships and Mode-of-Action of Antimic...
Next Document: Genetic incorporation of unnatural amino acids into proteins in yeast.