Document Detail

Experimental ischemic wounds: correlation of cell proliferation and insulin-like growth factor I expression and its modification by different local IGF-I release systems.
MedLine Citation:
PMID:  15953047     Owner:  NLM     Status:  MEDLINE    
We investigated cell proliferation and local insulin-like growth factor-I (IGF-I) expression in ischemic wounds after topical application of IGF-I through different delivery systems. IGF-I dressings were fabricated from an IGF-I containing polyvinyl alcohol film placed on a standard hydrogel dressing. In vitro, the release of IGF-I from this dressing was assessed by enzyme-linked immunosorbent assay. For animal experiments, a standardized ischemic skin flap containing a full-thickness wound was created on the back of male Sprague-Dawley rats. An identical wound outside the flap served as control. We initially investigated intracutaneous pO2 (p(ti)O2), cell proliferation, and local IGF-I expression. In a second setting, wounds were treated either with IGF-I dissolved in methylcellulose gel or with an IGF-I dressing, and ulcer size and cell proliferation were assessed. In vitro, approximately 60% of IGF-I was released from the IGF-I dressing, compared to a 97% release from methylcellulose gel. In vivo, ischemic wounds showed less cell proliferation and decreased IGF-I expression than nonischemic wounds. A lower local p(ti)O2 correlated with larger wound size, less cell proliferation, and decreased IGF-I expression. Ulcer size was reduced after treatment with either IGF-I dressing or methylcellulose gel. However, cell proliferation only increased after treatment with IGF-I dressing, but not after methylcellulose gel treatment. We conclude that IGF-I expression is decreased in ischemic wounds and correlates with low cell proliferation. This can be reversed by local IGF-I application, but the efficacy of treatment depends on the delivery system.
Stefan Beckert; Helmut Hierlemann; Nicole Müschenborn; Maria Witte; Michael Ranke; Stephan Coerper
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  13     ISSN:  1067-1927     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-06-14     Completed Date:  2005-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  278-83     Citation Subset:  IM    
Department of General Surgery, University of Tübingen, Germany.
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MeSH Terms
Administration, Topical
Bandages, Hydrocolloid
Cell Proliferation / drug effects
Drug Delivery Systems / methods
Growth Substances / administration & dosage*
Insulin-Like Growth Factor I / administration & dosage*
Ischemia / complications*
Methylcellulose / therapeutic use
Polyvinyl Alcohol / therapeutic use
Skin Ulcer / etiology,  physiopathology,  therapy*
Wound Healing / drug effects*
Wounds and Injuries / etiology,  physiopathology,  therapy
Reg. No./Substance:
0/Growth Substances; 67763-96-6/Insulin-Like Growth Factor I; 9002-89-5/Polyvinyl Alcohol; 9004-67-5/Methylcellulose

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