Document Detail


Experimental infection of calves with Leptospira interrogans serovar hardjo: conjunctival versus intravenous route of exposure.
MedLine Citation:
PMID:  3994099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eight-month-old calves, housed under maximum isolation, were exposed to pathogenic Leptospira interrogans serovar hardjo by the conjunctival route or IV. One calf served as an unexposed control. Infection was monitored serologically (microscopic agglutination test and enzyme-linked immunosorbent assay; ELISA) and by leptospiral culture isolation from periodic urine samples and from the kidneys, epididymides, and aqueous humor collected at slaughter. Microscopic agglutination test titers of greater than or equal to 1:40 were detected among all IV exposed calves at postinoculation day (PID) 7 and among conjunctival exposed calves at PID 14. By ELISA, all IV exposed calves were positive by PID 3, whereas conjunctival exposed calves were positive at PID 14. The ELISA was more sensitive for the detection of antibodies against leptospires in cattle. Leptospires were isolated from the urine of 4 calves and from the kidney of 3 calves exposed by the conjunctival route, but not from IV exposed calves. The results indicated that the conjunctival route of exposure was a more natural and successful route for experimental infection of cattle with serovar hardjo.
Authors:
A B Thiermann; A L Handsaker
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of veterinary research     Volume:  46     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-06-12     Completed Date:  1985-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  329-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agglutination Tests / veterinary
Animals
Antibodies, Bacterial / analysis
Cattle
Cattle Diseases / immunology,  transmission*
Conjunctiva / microbiology*
Enzyme-Linked Immunosorbent Assay
Injections, Intravenous / veterinary
Kidney / microbiology
Leptospira interrogans / immunology,  isolation & purification
Urine / microbiology
Weil Disease / immunology,  transmission,  veterinary*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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