| Experimental identification of microRNA-140 targets by silencing and overexpressing miR-140. | |
| | |
MedLine Citation:
|
PMID: 18945805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
MicroRNAs (miRNAs) are short noncoding RNA molecules regulating the expression of mRNAs. Target identification of miRNAs is computationally difficult due to the relatively low homology between miRNAs and their targets. We present here an experimental approach to target identification where the cartilage-specific miR-140 was overexpressed and silenced in cells it is normally expressed in separate experiments. Expression of mRNAs was profiled in both experiments and the intersection of mRNAs repressed by miR-140 overexpression and derepressed by silencing of miR-140 was identified. The intersection contained only 49 genes, although both treatments affected the accumulation of hundreds of mRNAs. These 49 genes showed a very strong enrichment for the miR-140 seed sequence implying that the approach is efficient and specific. Twenty-one of these 49 genes were predicted to be direct targets based on the presence of the seed sequence. Interestingly, none of these were predicted by the published target prediction methods we used. One of the potential target mRNAs, Cxcl12, was experimentally validated by Northern blot analysis and a luciferase reporter assay. |
| | |
Authors:
|
Francisco Esteban Nicolas; Helio Pais; Frank Schwach; Morten Lindow; Sakari Kauppinen; Vincent Moulton; Tamas Dalmay |
Related Documents
:
|
20371515 - Numerical modelling of microrna-mediated mrna decay identifies novel mechanism of micro... 18162065 - Effect of alcohol on mir-212 expression in intestinal epithelial cells and its potentia... 16211225 - Synthetic microrna and double-stranded rna targeting the 3'-untranslated region of her-... 20308325 - Ephrin-b1 reverse signaling controls a posttranscriptional feedback mechanism via mir-124. 7649105 - Developmental regulation of steroidogenic enzyme gene expression in the periimplantatio... 9160875 - Cardiac calcium release channel (ryanodine receptor) in control and cardiomyopathic hum... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-22 |
Journal Detail:
|
Title: RNA (New York, N.Y.) Volume: 14 ISSN: 1469-9001 ISO Abbreviation: RNA Publication Date: 2008 Dec |
Date Detail:
|
Created Date: 2008-12-05 Completed Date: 2008-12-24 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
|
Languages: eng Pagination: 2513-20 Citation Subset: IM |
Affiliation:
|
School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Chemokine CXCL12 / genetics Chickens Fibroblasts Mice MicroRNAs / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Chemokine CXCL12; 0/MicroRNAs |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Stabilization of RNA during laser capture microdissection by performing experiments under argon atmo...
Next Document: The RNA WikiProject: community annotation of RNA families.