| Experimental freeze-preservation of chondrocytes. | |
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MedLine Citation:
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PMID: 4017327 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Preservation of articular cartilage chondrocytes is currently being investigated in conjunction with storage and transplantation of osteochondral allografts. Studies on isolated chondrocytes have suggested that viability and function may be retained following freeze-preservation. Up to 90% of frozen isolated cells will survive, and these cells are capable of producing proteoglycans in culture. Results of freezing chondrocytes in a matrix have not been as successful, with viability ranging from 0% to 50%. Inconsistent results are thought to be due to the matrix and associated problems such as poor penetration of the cryopreservative, an unequal rate of cooling, and a high water content. Studies are in progress in an attempt to produce a satisfactory method of freeze-preservation that will promote normal functioning of chondrocytes after transplantation in an articular cartilage matrix. |
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Authors:
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W W Tomford; G P Duff; H J Mankin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Clinical orthopaedics and related research Volume: - ISSN: 0009-921X ISO Abbreviation: Clin. Orthop. Relat. Res. Publication Date: 1985 Jul-Aug |
Date Detail:
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Created Date: 1985-09-19 Completed Date: 1985-09-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0075674 Medline TA: Clin Orthop Relat Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 11-4 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage / cytology*, drug effects Cattle Cell Survival / drug effects Dimethyl Sulfoxide / pharmacology Freezing Tissue Preservation / methods |
| Grant Support | |
ID/Acronym/Agency:
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21896-04//PHS HHS |
| Chemical | |
Reg. No./Substance:
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67-68-5/Dimethyl Sulfoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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