Document Detail


Experimental and field studies on the suitability of raccoons (Procyon lotor) as hosts for tick-borne pathogens.
MedLine Citation:
PMID:  18429696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the experimental susceptibility and natural exposure of raccoons (Procyon lotor) to five tick-borne pathogens of human and veterinary importance, Ehrlichia canis, E. chaffeensis, E. ewingii, Anaplasma phagocytophilum (ApVariant 1 and Ap-ha HGE-1 strains), and Borrelia lonestari. Infections were assessed by polymerase chain reaction (PCR), indirect fluorescent antibody (IFA) testing, and/or culture isolation methods for at least 30 days postinoculation (DPI). Two E. chaffeensis-inoculated raccoons seroconverted and were transiently PCR positive. One raccoon was culture positive. Laboratory raised Amblyomma americanum nymphs fed on a third infected raccoon failed to become infected. Two A. phagocytophilum (HGE-1)-inoculated raccoons became PCR positive and seroconverted. Both remained positive for at least 74 DPI. In contrast, raccoons inoculated with A. phagocytophilum (Ap-Variant 1) were only transiently PCR positive and only seroconverted with low titers. No evidence of infection was observed for E. ewingii- and B. lonestari-inoculated raccoons. Only one E. canis-inoculated raccoon was PCR positive 3 DPI. Serologic testing of wild raccoons from five populations (3 infested with ticks) in Georgia and Florida showed antibodies reactive with E. chaffeensis in the 3 tick-infested populations (range of 30%-46%), E. canis in the same three populations (8%-23%), A. phagocytophilum in a single raccoon from Florida (12%), and Borrelia spp. in all 5 populations (8%-53%). All raccoons were PCR negative for tick-borne pathogens. These data suggest that raccoons are likely not important reservoirs of E. canis, E. ewingii, or B. lonestari. However, raccoons are experimentally susceptible and naturally exposed to E. chaffeensis, and these data support the previous finding that raccoons may be involved in the natural history of A. phagocytophilum.
Authors:
Michael J Yabsley; Staci M Murphy; M Page Luttrell; Susan E Little; Robert F Massung; David E Stallknecht; Lisa A Conti; Carina G M Blackmore; Lance A Durden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Vector borne and zoonotic diseases (Larchmont, N.Y.)     Volume:  8     ISSN:  1557-7759     ISO Abbreviation:  Vector Borne Zoonotic Dis.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-09-01     Completed Date:  2008-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965525     Medline TA:  Vector Borne Zoonotic Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  491-503     Citation Subset:  IM    
Affiliation:
Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602, USA. myabsley@uga.edu
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MeSH Terms
Descriptor/Qualifier:
Anaplasma phagocytophilum / isolation & purification*
Animals
Borrelia / isolation & purification*
Borrelia Infections / epidemiology,  microbiology,  veterinary
Disease Reservoirs / microbiology*
Ehrlichia / isolation & purification*
Ehrlichiosis / epidemiology,  microbiology,  veterinary
Florida / epidemiology
Georgia / epidemiology
Humans
Raccoons / microbiology*
Tick-Borne Diseases / epidemiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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