Document Detail

Experimental evidence showing that no mitotically active female germline progenitors exist in postnatal mouse ovaries.
MedLine Citation:
PMID:  22778414     Owner:  NLM     Status:  MEDLINE    
It has been generally accepted for more than half a century that, in most mammalian species, oocytes cannot renew themselves in postnatal or adult life, and that the number of oocytes is already fixed in fetal or neonatal ovaries. This assumption, however, has been challenged over the past decade. In this study, we have taken an endogenous genetic approach to this question and generated a multiple fluorescent Rosa26(rbw/+);Ddx4-Cre germline reporter mouse model for in vivo and in vitro tracing of the development of female germline cell lineage. Through live cell imaging and de novo folliculogenesis experiments, we show that the Ddx4-expressing cells from postnatal mouse ovaries did not enter mitosis, nor did they contribute to oocytes during de novo folliculogenesis. Our results provide evidence that supports the traditional view that no postnatal follicular renewal occurs in mammals, and no mitotically active Ddx4-expressing female germline progenitors exist in postnatal mouse ovaries.
Hua Zhang; Wenjing Zheng; Yan Shen; Deepak Adhikari; Hiroo Ueno; Kui Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-09
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-08-01     Completed Date:  2012-10-26     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12580-5     Citation Subset:  IM    
Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30, Gothenburg, Sweden.
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MeSH Terms
DEAD-box RNA Helicases / biosynthesis,  genetics
Gene Expression Regulation / physiology
Genes, Reporter
Mice, Transgenic
Mitosis / physiology*
Oocytes / cytology*,  metabolism
Ovarian Follicle / cytology*,  growth & development*,  metabolism
Reg. No./Substance:
EC 3.6.1.-/DEAD-box RNA Helicases; EC 3.6.1.-/Ddx4 protein, mouse

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