Document Detail

Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns.
MedLine Citation:
PMID:  1503214     Owner:  NLM     Status:  MEDLINE    
The band dispersion phenomena in capillary zone electrophoresis (CZE) using untreated and surface-treated open tubular and gel-filled capillaries were experimentally evaluated, with emphasis on small capillary diameters (10-100 microns). Laser-induced fluorescence detection was used for high-sensitivity detection of the isoindoles originated from model amino acids. The plots of plate height vs electric field strength were generated for different column radii and compared with a theoretical model for CZE. In addition to the diffusion-controlled band dispersion in the relatively low electric field range, adsorptive interactions between a solute and the capillary wall may play a certain role in band-broadening. The sorption-desorption kinetics become important with increasing electric field strength. Thermal effects appear to contribute little to band-broadening in relatively small capillaries (less than 50-microns i.d.) within normal operating voltages (less than 30 kV), but could become significant in capillaries with larger bores (greater than 75-microns i.d.). With gel-filled capillaries of small diameters (less than 50-microns i.d.), diffusion processes can be minimized. In addition, thermal effects do not appear critical in such columns at reasonable voltages.
J Liu; V Dolnik; Y Z Hsieh; M Novotny
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  64     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-09-14     Completed Date:  1992-09-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1328-36     Citation Subset:  IM    
Department of Chemistry, Indiana University, Bloomington 47405.
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MeSH Terms
Electrophoresis, Polyacrylamide Gel / instrumentation,  methods*
Grant Support

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