| Experimental diabetes modulates collagen remodelling of joints in rats. | |
| | |
MedLine Citation:
|
PMID: 23018246 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The aim of this study was to evaluate extracellular matrix components in articular cartilage, ligaments and synovia in an experimental model of diabetes. Young Wistar rats were divided into a streptozotocin-induced (STZ; 35 mg/kg) diabetic group (DG; n=15) and a control group (CG; n=15). Weight, blood glucose and plasma anti-carboxymethyllysine were measured 70 days after STZ infusions. Knee joints, patellar ligaments, and lateral and medial collateral ligaments were isolated and stained with hematoxylin-eosin and Picrosirius. The total collagen content was determined by morphometry. Immunofluorescence was employed to evaluate types I, III, and V collagen in ligaments and synovial tissues and types II and XI collagen in cartilage. Results: Higher blood glucose levels and plasma anti-carboxymethyllysine were observed in DG rats when compared to those in CG rats. The final weight was significantly lower in the DG rats than in the CG rats. Histomorphometric evaluation depicted a small quantity of collagen fibers in ligaments and articular cartilage in DG rats, as well as increased collagen in synovial tissue. There was a decrease in cartilage proteoglycans in DG rats when compared with CG rats. Immunofluorescence staining revealed an increase of collagen III and V in ligaments, collagen XI in cartilage, and collagen I in synovial tissue of DG rats compared with CG rats. Conclusion: The ligaments, cartilage and synovia are highly affected following STZ-induced diabetes in rats, due the remodeling of collagen types in these tissues. This process may promote the degradation of the extracellular matrix, thus compromising joint function. Our data may help to better understand the pathogenesis of joint involvement related to diabetes. |
| | |
Authors:
|
S A Atayde; N H Yoshinari; D P Nascimento; S Catanozi; P C Andrade; A P P Velosa; E R Parra; M Passarelli; E R Nakandakare; V L Capelozzi; W R Teodoro |
Related Documents
:
|
11247536 - Effect of urea and pantothenol on the permeation of progesterone through excised rat sk... 19280376 - Effect of chemical enhancers on percutaneous absorption of daphnetin in isopropyl myris... 1449476 - Substrate preference of metalloproteinases secreted by ts-110 moloney murine sarcoma vi... 12070496 - The effect of fibrin clot on healing rat supraspinatus tendon defects. 7271366 - Effect of gossypol on boar spermatozoa in vitro. 3583156 - Lack of hepatic microsomal metabolism of deoxynivalenol and its metabolite, dom-1. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Histology and histopathology Volume: 27 ISSN: 1699-5848 ISO Abbreviation: Histol. Histopathol. Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-09-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
|
Languages: eng Pagination: 1471-9 Citation Subset: IM |
Affiliation:
|
Rheumatology (LIM/17) Division, Medical School of the University of Sao Paulo. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Distribution of exogenous metallothionein following intraperitoneal and intramuscular injection of m...
Next Document: Clinicopathologic features of molecular subtypes of triple negative breast cancer based on immunohis...