| Experimental determination of net protein charge and A(tot) and K(a) of nonvolatile buffers in canine plasma. | |
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MedLine Citation:
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PMID: 16095167 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acid-base abnormalities frequently are present in sick dogs. The mechanism for an acid-base disturbance can be determined with the simplified strong ion approach, which requires accurate values for the total concentration of plasma nonvolatile buffers (A(tot)) and the effective dissociation constant for plasma weak acids (K(a)). The aims of this study were to experimentally determine A(tot) and K(a) values for canine plasma. Plasma was harvested from 10 healthy dogs; the concentrations of quantitatively important strong ions (Na+, K+, Ca2+, Mg2+, Cl-, L-lactate) and nonvolatile buffer ions (total protein, albumin, phosphate) were determined; and the plasma was tonometered with CO2 at 37 degrees C. Strong ion difference (SID) was calculated from the measured strong ion concentrations, and nonlinear regression was used to estimate values for A(tot) and K(a), which were validated with data from an in vitro and in vivo study. Mean (+/- SD) values for canine plasma were A(tot) = (17.4 +/- 8.6) mM (equivalent to 0.273 mmol/g of total protein or 0.469 mmol/g of albumin); K(a) = (0.17 +/- 0.11) x 10(-7); pK(a) = 7.77. The calculated SID for normal canine plasma (pH = 7.40; P(CO2) = 37 mm Hg; [total protein] = 64 g/L) was 27 mEq/L. The net protein charge for normal canine plasma was 0.25 mEq/g of total protein or 0.42 mEq/g of albumin. Application of the experimentally determined values for A(tot), K(a), and net protein charge should improve understanding of the mechanism for complex acid-base disturbances in dogs. |
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Authors:
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Peter D Constable; Henry R Stämpfli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine Volume: 19 ISSN: 0891-6640 ISO Abbreviation: J. Vet. Intern. Med. Publication Date: 2005 Jul-Aug |
Date Detail:
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Created Date: 2005-08-12 Completed Date: 2005-09-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8708660 Medline TA: J Vet Intern Med Country: United States |
Other Details:
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Languages: eng Pagination: 507-14 Citation Subset: IM |
Affiliation:
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Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA. constable@uiuc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid-Base Equilibrium* Acids / blood* Animals Blood Proteins / chemistry* Buffers Cats / blood Cattle / blood Dogs / blood* Female Horses / blood Humans Hydrogen-Ion Concentration Kinetics Male Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Blood Proteins; 0/Buffers |
| Comments/Corrections | |
Comment In:
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J Vet Intern Med. 2005 Jul-Aug;19(4):489-90
[PMID:
16095164
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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