Document Detail

Experimental cell transplantation therapy in rat myocardial infarction model including nude rat preparation.
MedLine Citation:
PMID:  20680815     Owner:  NLM     Status:  MEDLINE    
As a novel potential therapeutic strategy for cardiac disease, cell transplantation therapy has been extensively investigated in experimental studies and clinical trials. Although encouraging results have been demonstrated, a number of critical questions still remain to be answered. For example, what kind of stem cell and how many cells should be used; what is the best time for cell transplantation after acute myocardial infarction; which delivery approach is better, intravenous injection or direct intramyocardial injection? Transplantation of cells derived from human tissues into experimental animals may elicit an immune rejection. Immunodeficient nude rats provide a useful myocardial infarction model for cell transplantation therapy studies. We introduce our detailed methods of direct intramyocardial injection of immature heart cells and stem cells into the myocardial infarction region of rats and nude rats. Careful maintenance under aseptic conditions and proper surgical technique are essential to improve the survival of immunodeficient rats after surgery.
Wangde Dai; Robert A Kloner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  660     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-03     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  99-109     Citation Subset:  IM    
Good Samaritan Hospital, The Heart Institute, Division of Cardiovascular Medicine of the Keck School of Medicine at University of Southern California, Los Angeles, CA, USA.
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MeSH Terms
Cell Line
Myocardial Infarction / therapy*
Myocytes, Cardiac / cytology,  transplantation
Rats, Nude
Rats, Sprague-Dawley
Tissue Therapy / methods*
Grant Support

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