| Experimental approach for an in vitro toxicity assay with non-aggregated quantum dots. | |
| | |
MedLine Citation:
|
PMID: 19465109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Engineered nanoparticles are increasingly used in consumer products. While the potential of these products hold great promise, it is not known what potential toxic effects these nanomaterials may have on human health. There is a need to develop affordable, systematic, short-term in vitro assays aimed at allowing rapid assessment of potential toxicity. The method reported in this paper describes a system in which the intestinal lining is mimicked (Caco-2 human intestinal cell line) and provides an environment in which quantum dots (QDs), and possibly other nanomaterials, can be applied. Transepithelial electrical resistance (TEER) measurements assessed whether the epithelial integrity was breached because of QD exposure. QDs were suspended in calcium/magnesium-free phosphate buffered saline to study non-aggregated QDs. To maintain cell integrity, normal cell culture conditions were retained below the epithelium to provide necessary nutrients and ions. Toxicity studies completed here show that the nanosized QDs coated with hydrophilic thioglycolate capping ligands purchased for these experiments caused disruption in the epithelium monolayer and cell death at 0.1mg/L of QDs. This toxicity was caused by the nano-size of the QDs rather than the cadmium ions or the sodium thioglycolate capping ligands. Aggregated QDs did not cause toxicity as measured by TEER. |
| | |
Authors:
|
Brian A Koeneman; Yang Zhang; Kiril Hristovski; Paul Westerhoff; Yongsheng Chen; John C Crittenden; David G Capco |
Related Documents
:
|
19850119 - Assay conditions can influence the outcome of cytotoxicity tests of nanomaterials: bett... 22619509 - Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold na... 17663539 - Specific plant terpenoids and lignoids possess potent antiviral activities against seve... 18958429 - Physicochemical studies of a newly synthesized molecule, 6-methyl-3-phenethyl-3,4-dihyd... 15684479 - Cytotoxicity of flavonoids toward cultured normal human cells. 15936999 - In vitro toxicity test of ethyl 2-cyanoacrylate, a tissue adhesive used in cardiovascul... 12901499 - Cartilage-like phenomenon in the anterior cruciate ligamenta. 20693009 - Assessment of membrane toxicity using [(3)h]arachidonic acid release in u937 cells. 18601079 - Cell growth on immobilized cell growth factor. 8. protein-free cell culture on insulin-... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-22 |
Journal Detail:
|
Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 23 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-07-06 Completed Date: 2009-10-13 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
|
Languages: eng Pagination: 955-62 Citation Subset: IM |
Affiliation:
|
Cellular and Molecular Biosciences Faculty, School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Caco-2 Cells Cadmium Compounds / chemistry Cell Death / drug effects Electric Impedance Humans Ligands Particle Size Quantum Dots* Tellurium / chemistry Thioglycolates / chemistry Toxicity Tests / methods* |
| Chemical | |
Reg. No./Substance:
|
0/Cadmium Compounds; 0/Ligands; 0/Thioglycolates; 1306-25-8/cadmium telluride; 13494-80-9/Tellurium; 68-11-1/2-mercaptoacetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neonatal ontogeny and neurotoxic effect of decabrominated diphenyl ether (PBDE 209) on levels of syn...
Next Document: AhR-mediated gene expression in the developing mouse telencephalon.