Document Detail

Experimental and Modeling Analysisof Synechocystis sp. PCC 6803 Growth.
MedLine Citation:
PMID:  22508451     Owner:  NLM     Status:  Publisher    
Background/Aims: The influence of different parameters such as temperature, irradiance, nitrate concentration, pH, and an external carbon source on Synechocystis PCC 6803 growth was evaluated. Methods: 4.5-ml cuvettes containing 2 ml of culture, a high-throughput system equivalent to batch cultures, were used with gas exchange ensured by the use of a Parafilm™ cover. The effect of the different variables on maximum growth was assessed by a multi-way statistical analysis. Results: Temperature and pH were identified as the key factors. It was observed that Synechocystis cells have a strong influence on the external pH. The optimal growth temperature was 33°C while light-saturating conditions were reached at 40 μE·m(-2)·s(-1). Conclusion: It was demonstrated that Synechocystis exhibits a marked difference in behavior between autotrophic and glucose-based mixotrophic conditions, and that nitrate concentrations did not have a significant influence, probably due to endogenous nitrogen reserves. Furthermore, a dynamic metabolic model of Synechocystis photosynthesis was developed to gain insights on the underlying mechanism enabling this cyanobacterium to control the levels of external pH. The model showed a coupled effect between the increase of the pH and ATP production which in turn allows a higher carbon fixation rate.
Miguel Lopo; Arnau Montagud; Emilio Navarro; Isabel Cunha; Andrea Zille; Pedro Fernández de Córdoba; Pedro Moradas-Ferreira; Paula Tamagnini; Javier F Urchueguía
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-14
Journal Detail:
Title:  Journal of molecular microbiology and biotechnology     Volume:  22     ISSN:  1660-2412     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892561     Medline TA:  J Mol Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  71-82     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
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