Document Detail


Experiment on the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles.
MedLine Citation:
PMID:  19929650     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to investigate the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles. Ultrasound (US) combined with phospholipid-based microbubbles (MB) was used to enhance the susceptibility of colon cancer cell line SWD-620 to anticancer drugs Topotecan hydrochloride (TOP). Experiments were designed to investigate the influence of main factors on cell viability and cell inhibition, such as US intensity, MB concentration, drug combination with MB, asynchronous action between US triggered cavitation and drug entering cell, MB particle size. US exposure for 10 sec with US probe power at 0.6 W/cm(2) had satisfied cell viability. Treated with US combined with 15% MB, cell viability maintained more than 85% and cell inhibition 86.16%. Under optimal US combined with MB, TOP showed much higher cell inhibition than that of only TOP group. Cell inhibition under short delayed time (<2 h) for TOP addition did not show obvious difference. In terms of MB particle size, the order of cell inhibition was: Mixture > Micron bubble part > Nanometer bubble part. US combined with MB can enhance the susceptibility of cancer cells to chemotherapeutic drug, which may provide a potential method for US-mediated tumor chemotherapy.
Authors:
Ying-Zheng Zhao; Hui-Sheng Gao; Zhi-Cai Zhou; Qin-Qin Tang; Cui-Tao Lu; Zhuo Jin; Ji-Lai Tian; Yan-Yan Xu; Xin-Qiao Tian; Lee Wang; Fan-Lei Kong; Xiao-Kun Li; Pin-Tong Huang; Hui-Liao He; Yan Wu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of drug targeting     Volume:  18     ISSN:  1029-2330     ISO Abbreviation:  J Drug Target     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312476     Medline TA:  J Drug Target     Country:  England    
Other Details:
Languages:  eng     Pagination:  430-7     Citation Subset:  IM    
Affiliation:
Pharmacy School, Wenzhou Medical College, Zhejiang Province, China. lctuua@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / administration & dosage*,  pharmacokinetics,  pharmacology
Cell Line, Tumor
Cell Survival / drug effects
Drug Delivery Systems / methods*
Humans
Microbubbles*
Phospholipids / chemistry
Sonication
Topotecan / administration & dosage*,  pharmacokinetics,  pharmacology
Ultrasonics*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Phospholipids; 123948-87-8/Topotecan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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