| Experiences with Citrate Rather Than Heparin as an Anticoagulant for 18F-FDG Labeling of Leukocytes. | |
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MedLine Citation:
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PMID: 22072700 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In all reported clinical studies with (18)F-FDG-labeled leukocytes, heparin was used as an anticoagulant during labeling. Theoretically, the substitution of heparin with citrate should be advantageous. METHODS: Blood from healthy controls was sampled in duplicate, anticoagulated with citrate or heparin, and labeled with (18)F-FDG, and the labeling yield was measured. Viability was checked with the trypan blue exclusion technique. Moreover, 4 in vivo PET/CT studies were performed after the reinjection of leukocytes labeled after citrate anticoagulation. RESULTS: The labeling yields obtained with citrate and heparin were not significantly different (P = 0.447). Viability was greater than or equal to 99%. The quality of the PET/CT studies was excellent. In the in vivo studies, the mean labeling yield was 78%-better than or equal to that reported with heparin as an anticoagulant. CONCLUSION: Citrate is at least as effective as heparin as an anticoagulant, does not (unlike heparin) increase granulocyte activation, and should be the preferred anticoagulant for (18)F-FDG labeling of leukocytes. |
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Authors:
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Charlotte Eldjarn; Merethe Wigen Andersen; Randi Moen Forfang; Signe E Hagve; Kjell Rootwelt |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-9 |
Journal Detail:
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Title: Journal of nuclear medicine technology Volume: - ISSN: 1535-5675 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0430303 Medline TA: J Nucl Med Technol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Department of Nuclear Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway; and. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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