| Expanding the scope of quantitative FRAP analysis. | |
| | |
MedLine Citation:
|
PMID: 19836405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this study, new mathematical models were developed for analysis of fluorescence recovery after photobleaching (FRAP) data to account for features not represented in previous analysis: conical photobleaching geometry, spatial variations in binding of fluorescent molecules, and directed transport of fluorescent molecules. To facilitate computations in conical geometry, a fast computational method for calculation of fluorescence recovery is presented. Two approximations are presented to aid in FRAP analysis when binding varies spatially, one applying to cases of relatively fast diffusion and slow binding and the other to binding of molecules to small cellular structures. Numerical results show that using a model that represents the influential physical processes and that is formulated in the appropriate geometry can substantially improve the accuracy of FRAP calculations. |
| | |
Authors:
|
Mark A Hallen; Anita T Layton |
Related Documents
:
|
1373445 - Studies with compounds that compete with tryptophan binding to rat hepatic nuclei. 3595865 - Interaction of seminalplasmin with chlortetracycline, a fluorescent chelate probe of ca2+. 19139975 - Incorporation of siderophore binding sites in a dipodal fluorescent sensor for fe(iii). 14714735 - Effect of ethylene glycol and polyethylene glycol on the acid-unfolded state of trypsin... 2820985 - Analysis of the kinetics of cyclic amp hydrolysis by the cyclic gmp-stimulated cyclic n... 12887295 - Introduction to mannan-binding lectin. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-15 |
Journal Detail:
|
Title: Journal of theoretical biology Volume: 262 ISSN: 1095-8541 ISO Abbreviation: J. Theor. Biol. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2009-11-30 Completed Date: 2010-01-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376342 Medline TA: J Theor Biol Country: England |
Other Details:
|
Languages: eng Pagination: 295-305 Citation Subset: IM |
Affiliation:
|
Duke University, Department of Mathematics, Durham, NC 27708, USA. mah43@duke.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Transport Computer Simulation Diffusion Fluorescence Recovery After Photobleaching / methods* Models, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Signal propagation in small-world biological networks with weak noise.
Next Document: The role of face shape and pigmentation in other-race face perception: an electrophysiological study...