| Expanding the protein catalogue in the proteome reference map of human breast cancer cells. | |
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MedLine Citation:
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PMID: 16526084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this report we present a catalogue of 162 proteins (including isoforms and variants) identified in a prototype of proteomic map of breast cancer cells. This work represents the prosecution of previous studies describing the protein complement of breast cancer cells of the line 8701-BC, which has been well characterized for several parameters, providing to be a useful model for the study of breast cancer-associated candidate biomarkers. In particular, 110 spots were identified ex novo by PMF, or validated following previous gel matching identification method; 30 were identified by N-terminal microsequencing and the remaining by gel matching with maps available from our former work. As a consequence of the expanded number of proteins, we have updated our previous classification extending the number of protein groups from 4 to 13. In order to facilitate comparative proteome studies of different kinds of breast cancers, in this report we provide the whole complement of proteins so far identified and grouped into the new classification. A consistent number of them were not described before in other proteomic maps of breast cancer cells or tissues, and therefore they represent a valuable contribution for breast cancer protein databases and for future application in basic and clinical researches. |
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Authors:
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Ida Pucci-Minafra; Patrizia Cancemi; Simona Fontana; Luigi Minafra; Salvatore Feo; Michel Becchi; Anne-Marie Freyria; Salvatore Minafra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteomics Volume: 6 ISSN: 1615-9853 ISO Abbreviation: Proteomics Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-18 Completed Date: 2006-06-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 2609-25 Citation Subset: IM |
Affiliation:
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Dipartimento di Oncologia Sperimentale ed Applicazioni Cliniche (DOSAC), Via San Lorenzo Colli, Palermo, Italy. idapucci@unipa.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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metabolism* Cell Line, Tumor Cytoskeleton / metabolism Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Neoplastic* Heat-Shock Proteins / chemistry Humans Oxidation-Reduction Proteome Proteomics / methods* RNA / chemistry Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tumor Markers, Biological |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins; 0/Proteome; 0/Tumor Markers, Biological; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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