Document Detail

Expanding the phenotype associated with FOXG1 mutations and in vivo FoxG1 chromatin-binding dynamics.
MedLine Citation:
PMID:  22091895     Owner:  NLM     Status:  MEDLINE    
Mutations in the Forkhead box G1 (FOXG1) gene, a brain specific transcriptional factor, are responsible for the congenital variant of Rett syndrome. Until now FOXG1 point mutations have been reported in 12 Rett patients. Recently seven additional patients have been reported with a quite homogeneous severe phenotype designated as the FOXG1 syndrome. Here we describe two unrelated patients with a de novo FOXG1 point mutation, p.Gln46X and p.Tyr400X, respectively, having a milder phenotype and sharing a distinctive facial appearance. Although FoxG1 action depends critically on its binding to chromatin, very little is known about the dynamics of this process. Using fluorescence recovery after photobleaching, we showed that most of the GFP-FoxG1 fusion protein associates reversibly to chromatin whereas the remaining fraction is bound irreversibly. Furthermore, we showed that the two pathologic derivatives of FoxG1 described in this paper present a dramatic alteration in chromatin affinity and irreversibly bound fraction in comparison with Ser323fsX325 mutant (associated with a severe phenotype) and wild type Foxg1 protein. Our observations suggest that alterations in the kinetics of FoxG1 binding to chromatin might contribute to the pathological effects of FOXG1 mutations.
R De Filippis; L Pancrazi; K Bjørgo; A Rosseto; T Kleefstra; E Grillo; A Panighini; F Cardarelli; I Meloni; F Ariani; M A Mencarelli; J Hayek; A Renieri; M Costa; F Mari
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-13
Journal Detail:
Title:  Clinical genetics     Volume:  82     ISSN:  1399-0004     ISO Abbreviation:  Clin. Genet.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-12     Completed Date:  2013-01-07     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0253664     Medline TA:  Clin Genet     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  395-403     Citation Subset:  IM    
Copyright Information:
© 2011 John Wiley & Sons A/S.
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MeSH Terms
Blotting, Western
Chromatin / metabolism*
Chromosomes, Human, Pair 15 / genetics*
DNA Methylation / genetics
Fluorescence Recovery After Photobleaching
Forkhead Transcription Factors / genetics*,  metabolism
Genetic Diseases, Inborn / genetics*,  pathology*
Microscopy, Fluorescence
Nerve Tissue Proteins / genetics*,  metabolism
Point Mutation / genetics
Grant Support
Reg. No./Substance:
0/Chromatin; 0/FOXG1 protein, human; 0/Forkhead Transcription Factors; 0/Nerve Tissue Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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