| Expanded numbers of circulating myeloid dendritic cells in patent human filarial infection reflect lower CCR1 expression. | |
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MedLine Citation:
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PMID: 20956349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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APC dysfunction has been postulated to mediate some of the parasite-specific T cell unresponsiveness seen in patent filarial infection. We have shown that live microfilariae of Brugia malayi induce caspase-dependent apoptosis in human monocyte-derived dendritic cells (DCs) in vitro. This study addresses whether apoptosis observed in vitro extends to patent filarial infections in humans and is reflected in the number of circulating myeloid DCs (mDCs; CD11c(-)CD123(lo)) in peripheral blood of infected microfilaremic individuals. Utilizing flow cytometry to identify DC subpopulations (mDCs and plasmacytoid DCs [pDCs]) based on expression of CD11c and CD123, we found a significant increase in numbers of circulating mDCs (CD11c(+)CD123(lo)) in filaria-infected individuals compared with uninfected controls from the same filaria-endemic region of Mali. Total numbers of pDCs, monocytes, and lymphocytes did not differ between the two groups. To investigate potential causes of differences in mDC numbers between the two groups, we assessed chemokine receptor expression on mDCs. Our data indicate that filaria-infected individuals had a lower percentage of circulating CCR1(+) mDCs and a higher percentage of circulating CCR5(+) mDCs and pDCs. Finally, live microfilariae of B. malayi were able to downregulate cell-surface expression of CCR1 on monocyte-derived DCs and diminish their calcium flux in response to stimulation by a CCR1 ligand. These findings suggest that microfilaria are capable of altering mDC migration through downregulation of expression of some chemokine receptors and their signaling functions. These observations have major implications for regulation of immune responses to these long-lived parasites. |
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Authors:
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Roshanak Tolouei Semnani; Lily Mahapatra; Benoit Dembele; Siaka Konate; Simon Metenou; Housseini Dolo; Michel E Coulibaly; Lamine Soumaoro; Siaka Y Coulibaly; Dramane Sanogo; Salif Seriba Doumbia; Abdallah A Diallo; Sekou F Traoré; Amy Klion; Thomas B Nutman; Siddhartha Mahanty |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural Date: 2010-10-18 |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 185 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-04 Completed Date: 2010-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 6364-72 Citation Subset: AIM; IM |
Affiliation:
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Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. rsemnani@niaid.nih.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Brugia malayi / immunology Cell Separation Chemotaxis, Leukocyte / immunology Clinical Trials as Topic Dendritic Cells / immunology*, metabolism Dipetalonema Infections / immunology Female Filariasis / blood, immunology* Flow Cytometry Humans Male Mansonelliasis / blood, immunology Middle Aged Myeloid Cells / immunology, metabolism Receptors, CCR1 / biosynthesis*, immunology Reverse Transcriptase Polymerase Chain Reaction Wuchereria bancrofti / immunology |
| Chemical | |
Reg. No./Substance:
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0/CCR1 protein, human; 0/Receptors, CCR1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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