Document Detail

Exopolysaccharides of Xanthomonas pathovar strains that infect rice and wheat crops.
MedLine Citation:
PMID:  11525629     Owner:  NLM     Status:  MEDLINE    
In order to understand the mode of action of the taxonomically related pathogens Xanthomonas campestris pv. translucens, Xanthomonas oryzae pv. oryzae, and Xanthomonas oryzae pv. oryzicola, which attack wheat and rice crops, we examined the compositional differences of their exopolysaccharides (EPSs). Maximum production of polysaccharide in shake cultures of these pathogens was observed between 24 and 72 h. X. campestris pv. translucens, the leaf streak pathogen of wheat, produced a higher amount of polysaccharide (46.97 microg/ml) at 72 h compared to X. oryzae pv. oryzae (42.02 microg/ml), the bacterial blight pathogen of rice, and X. oryzae pv. oryzicola (41.91 microg/ml), the bacterial leaf streak pathogen of rice. Infrared (FTIR) spectra suggested that the polysaccharides of all three Xanthomonas pathovar strains have an -OH group with intermolecular hydrogen bonding, a C-H group of methyl alkanes, an aldehyde (RCHO) group, a C=C or C=O group, and a C-O group. FTIR spectra also revealed the presence of an acid anhydride group in X. oryzae pv. oryzae, a secondary aromatic or aliphatic amine group in X. campestris pv. translucens, and a primary aromatic or aliphatic amine group in X. oryzae pv. oryzae and X. oryzae pv. oryzicola. Nuclear magnetic resonance (NMR) spectra revealed the presence of unsubstituted sugars, an acetyl amine of hexose or pentose, and a beta-anomeric carbon of hexose or pentose in the polysaccharides of all bacteria. NMR spectra also identified the alpha-anomeric carbon of hexose or pentose in all strains, and a branching at the fourth carbon of the sugar only in X. campestris pv. translucens; the presence of an uronic acid molecule (acid anhydride group) in X. oryzae pv. oryzae; and a deoxy sugar, rhamnose, in X. oryzae pv. oryzicola.
R Sunish Kumar; N Sakthivel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  55     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-08-29     Completed Date:  2002-01-17     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  782-6     Citation Subset:  IM    
Department of Biotechnology, Pondicherry University, Kalapet, India.
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MeSH Terms
Hydrogen Bonding
Magnetic Resonance Spectroscopy
Oryza sativa / microbiology*
Polysaccharides, Bacterial / biosynthesis*,  chemistry*
Spectroscopy, Fourier Transform Infrared
Triticum / microbiology*
Xanthomonas / metabolism*
Xanthomonas campestris / metabolism
Reg. No./Substance:
0/Polysaccharides, Bacterial

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