| Exopolysaccharides of Xanthomonas pathovar strains that infect rice and wheat crops. | |
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MedLine Citation:
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PMID: 11525629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to understand the mode of action of the taxonomically related pathogens Xanthomonas campestris pv. translucens, Xanthomonas oryzae pv. oryzae, and Xanthomonas oryzae pv. oryzicola, which attack wheat and rice crops, we examined the compositional differences of their exopolysaccharides (EPSs). Maximum production of polysaccharide in shake cultures of these pathogens was observed between 24 and 72 h. X. campestris pv. translucens, the leaf streak pathogen of wheat, produced a higher amount of polysaccharide (46.97 microg/ml) at 72 h compared to X. oryzae pv. oryzae (42.02 microg/ml), the bacterial blight pathogen of rice, and X. oryzae pv. oryzicola (41.91 microg/ml), the bacterial leaf streak pathogen of rice. Infrared (FTIR) spectra suggested that the polysaccharides of all three Xanthomonas pathovar strains have an -OH group with intermolecular hydrogen bonding, a C-H group of methyl alkanes, an aldehyde (RCHO) group, a C=C or C=O group, and a C-O group. FTIR spectra also revealed the presence of an acid anhydride group in X. oryzae pv. oryzae, a secondary aromatic or aliphatic amine group in X. campestris pv. translucens, and a primary aromatic or aliphatic amine group in X. oryzae pv. oryzae and X. oryzae pv. oryzicola. Nuclear magnetic resonance (NMR) spectra revealed the presence of unsubstituted sugars, an acetyl amine of hexose or pentose, and a beta-anomeric carbon of hexose or pentose in the polysaccharides of all bacteria. NMR spectra also identified the alpha-anomeric carbon of hexose or pentose in all strains, and a branching at the fourth carbon of the sugar only in X. campestris pv. translucens; the presence of an uronic acid molecule (acid anhydride group) in X. oryzae pv. oryzae; and a deoxy sugar, rhamnose, in X. oryzae pv. oryzicola. |
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Authors:
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R Sunish Kumar; N Sakthivel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 55 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-08-29 Completed Date: 2002-01-17 Revised Date: 2002-11-01 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 782-6 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Pondicherry University, Kalapet, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Hydrogen Bonding Magnetic Resonance Spectroscopy Oryza sativa / microbiology* Polysaccharides, Bacterial / biosynthesis*, chemistry* Spectroscopy, Fourier Transform Infrared Triticum / microbiology* Xanthomonas / metabolism* Xanthomonas campestris / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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