| Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties. | |
| | |
MedLine Citation:
|
PMID: 20884069 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Exopolysaccharides (EPSs) are a metabolite of probiotics which have gained wide interest recently, but little is known about their function. EPS was isolated from Bifidobacterium longum BCRC 14634 and sterilized by 0.22 μm filter. The proliferation of J77A.1 macrophages and their secretion of the anti-inflammatory cytokine interleukin-10 (IL-10) was elevated after treatment with heat-killed B. longum or 5 μg/mL EPS. The endotoxin, lipopolysaccharides (LPS), a potent inducer of pro-inflammatory cytokine tumor necrosis factor-α (TNF-α), significantly suppressed the growth of J77A.1 cells, and induced the secretion of TNF-α from J774A.1 cells. Furthermore, 24h pretreatment with 5 μg/ml EPS suppressed 100 ng/ml LPS-induced cell growth inhibition and release of TNF-α from J774A.1 cells. Additional experiments showed that 80 μg/mL EPS had antimicrobial activity against 7 species of food-spoilage and infection bacteria. Our results suggest that EPS from B. longum might be useful as a mild immune modulator for macrophages, contributing to the capacity of B. longum to fight against gastrointestinal infections, and even some food-spoilage microbe. |
| | |
Authors:
|
Ming-Hsiu Wu; Tzu-Ming Pan; Yu-Jen Wu; Sue-Joan Chang; Ming-Song Chang; Chun-Yi Hu |
Related Documents
:
|
12432449 - In vivo response of neutrophils and epithelial cells to lipopolysaccharide injected int... 6183219 - Silica enhancement of murine endotoxin sensitivity. 14966369 - Glossogyne tenuifolia acts to inhibit inflammatory mediator production in a macrophage ... 21031629 - Active principles of grindelia robusta exert antiinflammatory properties in a macrophag... 20019359 - Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex. 3087999 - Effects of bacterial lipopolysaccharide on protein synthesis in murine peritoneal macro... 17545099 - Effects of saussurea lappa roots extract in ethanol on leukocyte phagocytic activity, l... 19564349 - Dendritic cells require a systemic type i interferon response to mature and induce cd4+... 321689 - Immunity to sporozoite-induced malaria infeciton in mice. i. the effect of immunization... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-15 |
Journal Detail:
|
Title: International journal of food microbiology Volume: 144 ISSN: 1879-3460 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-11-09 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 104-10 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Department of Nutrition and Health Science, Fooyin University, Kaohsiung County, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A survey on the latest NICE guidance for diagnosis of chest pain: A challenge to NICE.
Next Document: Inactivation of Staphylococcus aureus and Escherichia coli by the synergistic action of high hydrost...