Document Detail

Exonuclease activities associated with DNA polymerases alpha and beta of the archaebacterium Halobacterium halobium.
MedLine Citation:
PMID:  1851484     Owner:  NLM     Status:  MEDLINE    
alpha-like and beta-like DNA polymerases have previously been isolated from a halophilic archaebacterium Halobacterium halobium. In this report, we show that the alpha-like DNA polymerase has an associated 3' to 5'-exonuclease activity which is specific for single-stranded DNA, sensitive to both aphidicolin and N-ethylmaleimide and dependent on high salt concentrations like the polymerase activity. As this DNA polymerase has been shown to contain a primase activity, it may be considered as the equivalent to both eukaryotic DNA polymerases alpha and delta. As shown by glycerol-gradient centrifugation and electrophoresis under denaturing conditions, the beta-like polymerase would appear to have a monomeric structure and comprise of a single 65-kDa polypeptide. This DNA polymerase has both 3' to 5'-exonuclease and 5' to 3'-exonuclease activities which, contrary to polymerase activity, are inhibited by high salt concentrations.
I Sorokine; K Ben-Mahrez; M Nakayama; M Kohiyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  197     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-06-20     Completed Date:  1991-06-20     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  781-4     Citation Subset:  IM    
Institut Jacques Monod, Centre National de la Recherche Scientifique, Université Paris 7, France.
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MeSH Terms
DNA Polymerase I / analysis*,  chemistry
DNA Polymerase II / analysis*,  chemistry
Exonucleases / analysis*
Halobacterium / enzymology*
Reg. No./Substance:
EC 2.7.7.-/DNA Polymerase I; EC 2.7.7.-/DNA Polymerase II; EC 3.1.-/Exonucleases

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