| Exonuclease activities associated with DNA polymerases alpha and beta of the archaebacterium Halobacterium halobium. | |
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MedLine Citation:
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PMID: 1851484 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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alpha-like and beta-like DNA polymerases have previously been isolated from a halophilic archaebacterium Halobacterium halobium. In this report, we show that the alpha-like DNA polymerase has an associated 3' to 5'-exonuclease activity which is specific for single-stranded DNA, sensitive to both aphidicolin and N-ethylmaleimide and dependent on high salt concentrations like the polymerase activity. As this DNA polymerase has been shown to contain a primase activity, it may be considered as the equivalent to both eukaryotic DNA polymerases alpha and delta. As shown by glycerol-gradient centrifugation and electrophoresis under denaturing conditions, the beta-like polymerase would appear to have a monomeric structure and comprise of a single 65-kDa polypeptide. This DNA polymerase has both 3' to 5'-exonuclease and 5' to 3'-exonuclease activities which, contrary to polymerase activity, are inhibited by high salt concentrations. |
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Authors:
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I Sorokine; K Ben-Mahrez; M Nakayama; M Kohiyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 197 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-06-20 Completed Date: 1991-06-20 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY |
Other Details:
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Languages: eng Pagination: 781-4 Citation Subset: IM |
Affiliation:
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Institut Jacques Monod, Centre National de la Recherche Scientifique, Université Paris 7, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA Polymerase I
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analysis*,
chemistry DNA Polymerase II / analysis*, chemistry Exonucleases / analysis* Halobacterium / enzymology* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.-/DNA Polymerase I; EC 2.7.7.-/DNA Polymerase II; EC 3.1.-/Exonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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