Document Detail


Exogenous thymosin beta4 prevents apoptosis in human intervertebral annulus cells in vitro.
MedLine Citation:
PMID:  20055734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Loss of cells in the human disc due to programmed cell death (apoptosis) is a major factor in the aging and degenerating human intervertebral disc. Our objective here was to determine if thymosin beta(4) (TB4), a small, multifunctional 5 kDa protein with diverse activities, might block apoptosis in human annulus cells cultured in monolayer or three-dimensional (3D) culture. Apoptosis was induced in vitro using hydrogen peroxide or serum starvation. Annulus cells were processed for identification of apoptotic cells using the TUNEL method. The percentage of apoptotic cells was determined by cell counts. Annulus cells also were treated with TB4 for determination of proliferation, and proteoglycan production was assessed using cell titer and 1,2 dimethylmethylamine (DMB) assays and histological staining. A significant reduction in disc cell apoptosis occurred after TB4 treatment. The percentage of cells undergoing apoptosis decreased significantly in TB4 treated cells in both apoptosis induction designs. TB4 exposure did not alter proteoglycan production as assessed by either DMB measurement or histological staining. Our results indicate the need for further studies of the anti-apoptotic effect of TB4 and suggest that TB4 may have therapeutic application in future biological therapies for disc degeneration.
Authors:
H Tapp; R Deepe; J A Ingram; E G Yarmola; M R Bubb; E N Hanley; H E Gruber
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  84     ISSN:  1473-7760     ISO Abbreviation:  Biotech Histochem     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-08     Completed Date:  2010-06-25     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  287-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Apoptosis / drug effects*
Extracellular Matrix
Female
Humans
Hydrogen Peroxide / pharmacology
In Situ Nick-End Labeling / methods
Intervertebral Disc / drug effects*
Lumbar Vertebrae / drug effects*
Male
Middle Aged
Proteoglycans / biosynthesis*,  pharmacology
Thymosin / pharmacology*
Grant Support
ID/Acronym/Agency:
5K25AR048918/AR/NIAMS NIH HHS; K25 AR048918/AR/NIAMS NIH HHS; K25 AR048918-05/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Proteoglycans; 61512-21-8/Thymosin; 77591-33-4/thymosin beta(4); BBX060AN9V/Hydrogen Peroxide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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