Document Detail

Exogenous sphingosine 1-phosphate induces neurite retraction possibly through a cell surface receptor in PC12 cells.
MedLine Citation:
PMID:  9388477     Owner:  NLM     Status:  MEDLINE    
Exogenous sphingosine 1-phosphate (S1P), like lysophosphatidic acid (LPA), induced neurite retraction or cell rounding in differentiated PC12 cells. The lysosphingolipid-induced shape change was detected at as low as 1 nM; however, a significant accumulation of intracellular S1P was not detected until 1 microM S1P was applied. Moreover, although exogenous sphingosine caused a significant increase in intracellular S1P by sphingosine kinase-catalyzed phosphorylation, the effect on the shape change was marginal. Exposure of the cells to the immobilized S1P in which the lipid was covalently linked to a glass carrier also resulted in the shape change. These results suggest that the exogenous S1P-induced shape change does not require uptake of the lipid into the cells but possibly requires interaction with a cell surface receptor in the neuronal cells.
K Sato; H Tomura; Y Igarashi; M Ui; F Okajima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  240     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-23     Completed Date:  1997-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  329-34     Citation Subset:  IM    
Laboratory of Signal Transduction, Gunma University, Maebashi, Japan. kosato@news
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MeSH Terms
Cell Differentiation
Cell Size / drug effects
Lysophospholipids / pharmacology
Neurites / physiology*
PC12 Cells
Phosphotransferases (Alcohol Group Acceptor) / metabolism
Receptors, Cell Surface / drug effects,  physiology*
Sphingosine / analogs & derivatives*,  metabolism,  pharmacology
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Lysophospholipids; 0/Receptors, Cell Surface; 123-78-4/Sphingosine; 16561-29-8/Tetradecanoylphorbol Acetate; 26993-30-6/sphingosine 1-phosphate; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.-/sphingosine kinase

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