| Exogenous human immunodeficiency virus-1 protein, tat, enhances replication of JC virus efficiently in neuroblastoma cell lines. | |
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MedLine Citation:
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PMID: 22337293 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The high incidence of progressive multifocal leukoencephalopathy (PML) among individuals with acquired immunodeficiency syndrome (AIDS) is similar to the incidence of other immunocompromised diseases. The pathogenic JC virus (JCV) with rearranged regulatory regions (PML-type) causes PML, a demyelinating disease in the brains of immunocompromised patients. In a previous study, Tat protein, encoded by human immunodeficiency virus type 1 (HIV-1), markedly enhanced the expression of a reporter gene under control of the JCV late promoter. In order to examine the enhancement of JCV replication by Tat protein, the neuroblastoma cell line IMR-32 was used because it enables IMR-32-adapted JCV. The extent of JCV replication in IMR-32 cells treated with Tat protein was significantly higher than that in untreated IMR-32 cells. The enhancement of JCV propagation by Tat protein was also examined using IMR-32-derived JCV producing (JCI) cells which continuously produce JCV. Treatment of JCI cells with Tat protein led to a significant increase in the titers of progeny viruses. It has also been shown that Tat protein leads to a decrease in the expression of purine-rich element binding protein α (Purα) as an important mediator of JCV replication in IMR-32 cells. Thus, it is probable that Tat protein enhances JCV replication in IMR-32 cells via the down-regulation of Purα expression and cell proliferation. To our knowledge, this is the first report that exogenous Tat protein enhances the replication of JCV efficiently in neuroblastoma cell lines. J. Med. Virol. 84:555-561, 2012. © 2011 Wiley Periodicals, Inc. |
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Authors:
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Souichi Nukuzuma; Masanori Kameoka; Shigeki Sugiura; Kazuo Nakamichi; Chiyoko Nukuzuma; Isao Miyoshi; Tsutomu Takegami |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of medical virology Volume: 84 ISSN: 1096-9071 ISO Abbreviation: J. Med. Virol. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-02-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: United States |
Other Details:
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Languages: eng Pagination: 555-61 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Department of Microbiology, Kobe Institute of Health, Chuo-ku, Kobe, Japan. s-nuku@gj8.so-net.ne.jp. |
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