| Exogenous high-mobility group box 1 protein prevents postinfarction adverse myocardial remodeling through TGF-β/Smad signaling pathway. | |
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MedLine Citation:
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PMID: 23355476 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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High-mobility group box 1 (HMGB1) has been reported to attenuate ventricular remodeling, but its mechanism remains mostly unresolved. Transforming growth factor-beta (TGF-β) is a crucial mediator in the pathogenesis of post-infarction remodeling. Our study focused on the effects of HMGB1 on ventricular remodeling, and explored whether or not these effects were depended upon the TGF-β signaling pathway. Rats underwent coronary artery ligation. An intramyocardium injection of phosphate buffered saline (PBS) with or without HMGB1 was administered 3 weeks after myocardial infarction (MI). At 4 weeks after the treatment, HMGB1 significantly increased the Left ventricular ejection fraction (LVEF) (P<0.05), decreased the left ventricular end diastolic dimension (LVEDD) (P<0.05), left ventricular end systolic dimension (LVESD) (P<0.05) and the infarct size (P<0.05) compared with control group. The expressions of collagen I, collagen III and tissue inhibitor of metalloproteinase 2 (TIMP2) were also decreased, while the matrix metalloproteinases 2 (MMP2) and MMP9 expressions were upregulated by HMGB1 injection (P<0.05) compared with control group. No effect on TIMP3 was observed. Furthermore, TGF-β1 and phosphor-Smad2 (p-Smad2) were significantly suppressed and Smad7 was increased in HMGB1-treated group (P<0.05) compared with control group, no effects on p-Smad3 and p-p38 were observed. HMGB1 also upregulated Smad 7 expression and decreased the level of collagen I on cardiac fibroblasts (P<0.05). Silencing of Smad7 gene by small interfering RNA abolished the fibrogenic effects of HMGB1 on cardiac fibroblasts (P<0.05). These finding suggested that HMGB1 injection modulated ventricular remodeling may function through the possible inhibition of TGF-β/Smad signaling pathway. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc. |
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Authors:
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Yiyu He; Xiaoya Zhou; Xiaoxin Zheng; Xuejun Jiang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-25 |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: - ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2013 Wiley Periodicals, Inc. |
Affiliation:
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Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, PR China; Cardiovascular Research Institute of Wuhan University. Wuhan, 430060, PR China. |
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