Document Detail


Exogenous ghrelin regulates proliferation and apoptosis in the hypotrophic gut mucosa of the rat.
MedLine Citation:
PMID:  20407078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ghrelin is the natural endogenous ligand for growth hormone secretagogue receptors. This peptide regulates energy homeostasis and expenditure and is a potential link between gut absorptive function and growth. We hypothesized that ghrelin may induce a proliferative and antiapoptotic action promoting the recovery of the hypotrophic gut mucosa. Therefore, the aim of the study was to determine the action of exogenous ghrelin following gut mucosal hypotrophia in rats fed an elemental diet. An elemental diet provides readily absorbable simple nutrients and is usually given to patients with absorptive dysfunction. Male Wistar rats (n = 48) were fed the elemental diet for one week to induce mucosal hypotrophy and then treated for another week with systemic ghrelin and pair-fed with either a normoproteic or hyperproteic isocaloric liquid diet. Another group received a standard diet instead of the elemental diet and served as control (normotrophy). The elemental diet induced intestinal hypotrophia characterized by decreased proliferation in the ileum and increased apoptosis in jejunum and ileum. Ghrelin administration restored normal levels of proliferation in the ileum and apoptosis in the jejunum, with partial apoptosis restoration in the ileum. Ghrelin levels in plasma and fundus were increased in all groups, although the highest levels were found in rats treated with exogenous ghrelin. Ghrelin administration has a positive effect in the hypotrophic gut, regulating both proliferation and apoptosis towards a physiological balance counteracting the negative changes induced by an elemental diet in the intestines.
Authors:
Ignacio A Gómez de Segura; María Teresa Vallejo-Cremades; Jesús Lomas; Miriam F Sánchez; María Isabel Caballero; Carlota Largo; Enrique De Miguel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  235     ISSN:  1535-3699     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-21     Completed Date:  2010-04-28     Revised Date:  2010-07-29    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  England    
Other Details:
Languages:  eng     Pagination:  463-9     Citation Subset:  IM    
Affiliation:
Department of Experimental Surgery, Hospital Universitario La Paz, Paseo de la Castellana, 261, 28046 Madrid, Spain. ialvarez@vet.ucm.es
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Cell Proliferation / drug effects*
Diet / veterinary
Ghrelin / metabolism,  pharmacology*
Insulin-Like Growth Factor I / metabolism
Intestinal Mucosa* / anatomy & histology,  drug effects,  pathology
Male
Random Allocation
Rats
Rats, Wistar
Receptors, Ghrelin / metabolism
Chemical
Reg. No./Substance:
0/Ghrelin; 0/Receptors, Ghrelin; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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