| Exogenous expression of hepatocyte growth factor (HGF) in rat striatum by naked plasmid DNA. | |
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MedLine Citation:
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PMID: 12573464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hepatocyte growth factor (HGF) is a heterodimeric protein and shows mitogenic and morphogenic activities toward a variety of epithelial cells. There has been no immunohistochemical evidences for naked DNA mediated transgene expression of HGF into central nervous system. We initially demonstrated a naked plasmid mediated expression of HGF into rat striatum. The immunofluorescence staining revealed that exogenous protein of human HGF was expressed at 7 days after plasmid injection (300 microg). Exogenous HGF was mainly expressed in reactive astrocytes according to dual-labeling staining of HGF and glial fibrillary acidic protein or S100. It is also demonstrated that c-met, specific receptor of HGF, was expressed in the injection site. Intensive expression of c-met was found in the site to which HGF encoded plasmid was injected. These evidences for the exogenous expression of HGF and its receptor c-met may implicate an application of naked plasmid mediated HGF for neuronal disease as well as the other neurotrophic factors. |
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Authors:
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Keishi Sekiguchi; Fumioki Yasuzumi; Ryuichi Morishita |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience research Volume: 45 ISSN: 0168-0102 ISO Abbreviation: Neurosci. Res. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-07 Completed Date: 2003-04-10 Revised Date: 2006-05-01 |
Medline Journal Info:
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Nlm Unique ID: 8500749 Medline TA: Neurosci Res Country: Ireland |
Other Details:
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Languages: eng Pagination: 173-80 Citation Subset: IM |
Affiliation:
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Second Department of Anatomy, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. k008703@med.u-ryukyu.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Beetles Corpus Striatum / chemistry, metabolism* Female Gene Expression Regulation / drug effects, physiology Gene Transfer Techniques Hepatocyte Growth Factor / analysis, biosynthesis*, genetics Humans Male Plasmids / genetics, pharmacology* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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67256-21-7/Hepatocyte Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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