Document Detail

Exogenous androstenedione induces formation of follicular cysts and premature luteinization of granulosa cells in the ovary.
MedLine Citation:
PMID:  19100535     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the effects of androstenedione on ovarian follicle development. DESIGN: Experimental study. SETTING: University research laboratory. ANIMAL(S): Female Wistar-Imamichi rats and BDF1 mice. INTERVENTION(S): Rats were injected with androstenedione. Ovarian follicles of mice were cultured in the presence of androstenedione. MAIN OUTCOME MEASURE(S): Ovarian morphology; ovarian cell types undergoing apoptosis; ovarian expression of cytochrome P450 aromatase (P450arom), cytochrome P450 side-chain cleavage (P450scc), and cyclin-dependent kinase inhibitor p27(kip1); serum levels of T, E(2), and P in rats; and ultrastructure of granulosa cells from cultured follicles of mice. RESULT(S): In androstenedione-treated rat ovaries, follicular cysts were formed, and apoptotic cells were found in the inner part of granulosa cell layers of antral follicles. Androstenedione administration down-regulated expression of P450arom but up-regulated expression of P450scc and p27(Kip1) in the granulosa cells of antral follicles. Serum T levels were significantly increased in androstenedione-treated rats. In mouse follicles exposed to androstenedione, the granulosa cells contained abundant lipid droplets and mitochondria with complex tubular cristae. CONCLUSION(S): Excess androgen enhances apoptosis in the inner part of granulosa cell layers of antral follicles, resulting in the formation of follicular cysts. It is also demonstrated that androgen stimulates premature luteinization of granulosa cells.
Yuki Okutsu; Masanori T Itoh; Noriyuki Takahashi; Bunpei Ishizuka
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Publication Detail:
Type:  Journal Article     Date:  2008-12-18
Journal Detail:
Title:  Fertility and sterility     Volume:  93     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  927-35     Citation Subset:  IM    
Copyright Information:
Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Sugao, Miya-mae-ku, Kawasaki 216-8511, Japan.
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MeSH Terms
Androstenedione / pharmacology*
Apoptosis / drug effects
Aromatase / metabolism
Cell Communication / drug effects
Cyclin-Dependent Kinase Inhibitor p27 / metabolism
Cytochrome P-450 Enzyme System / metabolism
Follicular Cyst / chemically induced*,  pathology
Granulosa Cells / drug effects,  ultrastructure
Luteinization / drug effects*
Mice, Inbred Strains
Microscopy, Electron, Transmission
Oocytes / drug effects,  ultrastructure
Organ Culture Techniques
Ovarian Follicle / cytology,  drug effects*,  growth & development*
Polycystic Ovary Syndrome / pathology
Rats, Wistar
Reg. No./Substance:
0/Cdkn1b protein, mouse; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 63-05-8/Androstenedione; 9035-51-2/Cytochrome P-450 Enzyme System; EC

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