| Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus. | |
| | |
MedLine Citation:
|
PMID: 21329968 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Rhinoviruses are the major cause of asthma exacerbations. Previous studies suggest that primary bronchial epithelial cells (PBECs) from asthmatic subjects are more susceptible to rhinovirus infection because of deficient IFN-β production. Although augmenting the innate immune response might provide a novel approach for treatment of virus-induced asthma exacerbations, the potential of IFN-β to modulate antiviral and proinflammatory responses in asthmatic epithelium is poorly characterized. OBJECTIVES: We sought to compare responses of PBECs from nonasthmatic and asthmatic subjects to exogenous IFN-β and test the inflammatory effects of IFN-β in response to rhinovirus infection. METHODS: PBECs were treated with IFN-β and infected with a low inoculum of human rhinovirus serotype 1B to simulate a natural viral infection. Expression of interferon-responsive genes and inflammatory responses were analyzed by using reverse transcription-quantitative real-time PCR, cytometric bead arrays, or both; viral titers were assessed by using the 50% tissue culture infection dose. RESULTS: Expression of IFN-β-stimulated antiviral genes was comparable in PBECs from nonasthmatic or asthmatic donors. Exogenous IFN-β significantly protected PBECs from asthmatic donors against rhinovirus infection by suppressing viral replication. Interferon-inducible protein 10 (IP-10), RANTES, and IL-6 release in response to rhinovirus infection was triggered only in PBECs from asthmatic donors. Although exogenous IFN-β alone stimulated some release of IP-10 (but not IL-6 or RANTES), it significantly reduced rhinovirus-induced IP-10, RANTES, and IL-6 expression when tested in combination with rhinovirus. CONCLUSIONS: PBECs from asthmatic donors have a normal antiviral response to exogenous IFN-β. The ability of IFN-β to suppress viral replication suggests that it might limit virus-induced exacerbations by shortening the duration of the inflammatory response. |
| | |
Authors:
|
Julie A Cakebread; Yunhe Xu; Chris Grainge; Valia Kehagia; Peter H Howarth; Stephen T Holgate; Donna E Davies |
Related Documents
:
|
7865348 - Cytokines and the host defense against listeria monocytogenes and salmonella typhimurium. 15944308 - Phosphatidylinositol-specific phospholipase c of bacillus anthracis down-modulates the ... 21217078 - The nerve growth factor reduces apobec3g synthesis and enhances hiv-1 transcription and... 2622818 - Maturation of resistance to salmonellosis in newly hatched chicks: inhibition by cyclos... 15389638 - Rapid induction of the intrinsic apoptotic pathway by l-glutamine starvation. 847288 - The effects of anti-inflammatory agents on skin tumor initiation and aryl hydrocarbon h... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-2-15 |
Journal Detail:
|
Title: The Journal of allergy and clinical immunology Volume: - ISSN: 1097-6825 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-2-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved. |
Affiliation:
|
Division of Infection, Inflammation and Immunity, University of Southampton School of Medicine, Southampton, United Kingdom and the National Institute for Health Research (NIHR) Respiratory Biomedical Research Unit, Southampton General Hospital. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phase II study of cisplatin plus cetuximab in advanced, recurrent, and previously treated cancers of...
Next Document: Vitamin D levels and food and environmental allergies in the United States: results from the Nationa...