| The Exocytosis of Fluorescent Nanodiamond and Its Use as a Long-Term Cell Tracker. | |
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MedLine Citation:
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PMID: 21997958 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Fluorescent nanodiamond (FND) has excellent biocompatibility and photostability, making it well suited for long-term labeling and tracking of cancer and stem cells. To prove the concept, the exocytosis of FND particles (size ≈100 nm) from three cell lines-HeLa cervical cancer cells, 3T3-L1 pre-adipocytes, and 489-2.1 multipotent stromal cells-is studied in detail. FND labeling is performed by incubating the cells in a serum-free medium containing 80 μg mL(-1) FND for 4 h. No significant alteration in growth or proliferation of the FND-labeled cells, including the multipotent stromal cells, is observed for up to 8 days. Flow cytometric analysis, in combination with parallel cell doubling-time measurements, indicates that there is little (≈15% or less) excretion of the endocytosed FND particles after 6 days of labeling for both HeLa and 489-2.1 cells, but exocytosis occurs more readily (up to 30%) for 3T3-L1 preadipocytes. A comparative experiment with FND and the widely used dye, carboxyfluorescein diacetate succinimidyl ester, demonstrates that the nanoparticle platform is a promising alternate probe for long-term cell labeling and tracking applications. |
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Authors:
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Chia-Yi Fang; V Vaijayanthimala; Chi-An Cheng; Shih-Hua Yeh; Ching-Fang Chang; Chung-Leung Li; Huan-Cheng Chang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-14 |
Journal Detail:
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Title: Small (Weinheim an der Bergstrasse, Germany) Volume: - ISSN: 1613-6829 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101235338 Medline TA: Small Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Institute of Atomic and Molecular Sciences, Academia Sinica Taipei 106, Taiwan; Department of Chemistry, National Taiwan University, Taipei 106, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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