| Exo1: a new chemical inhibitor of the exocytic pathway. | |
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MedLine Citation:
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PMID: 12738886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A phenotypic screen was used to search for drug-like molecules that can interfere with specific steps in membrane traffic. 2-(4-Fluorobenzoylamino)-benzoic acid methyl ester (Exo1), identified in this screen, induces a rapid collapse of the Golgi to the endoplasmic reticulum, thus acutely inhibiting the traffic emanating from the endoplasmic reticulum. Like Brefeldin A (BFA), Exo1 induces the rapid release of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less effect on the organization of the trans-Golgi network. Our data indicate that Exo1 acts by a different mechanism from BFA. Unlike BFA, Exo1 does not induce the ADP-ribosylation of CtBP/Bars50 and does not interfere with the activity of guanine nucleotide exchange factors specific for Golgi-based ARFs. Thus, Exo1 allows the fatty acid exchange activity of Bars50 to be distinguished from ARF1 activity in the control of Golgi tubulation. |
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Authors:
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Yan Feng; Sidney Yu; Troy K R Lasell; Ashutosh P Jadhav; Eric Macia; Pierre Chardin; Paul Melancon; Michael Roth; Timothy Mitchison; Tomas Kirchhausen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2003-05-08 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 100 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-28 Completed Date: 2003-07-16 Revised Date: 2012-01-05 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 6469-74 Citation Subset: IM |
Affiliation:
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Institute of Chemistry and Cell Biology, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. yfeng@hms.harvard.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate Ribose
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metabolism Benzamides / chemistry, pharmacology* Benzoates / chemistry, pharmacology* Brefeldin A / pharmacology Cell Line Exocytosis* Golgi Apparatus / metabolism Guanine Nucleotide Exchange Factors / metabolism Microscopy, Fluorescence Phenotype |
| Grant Support | |
ID/Acronym/Agency:
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P01 GM62566-02/GM/NIGMS NIH HHS; R01 GM36548-17/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/2-(4-fluorobenzoylamino)benzoic acid methyl ester; 0/Benzamides; 0/Benzoates; 0/Guanine Nucleotide Exchange Factors; 20350-15-6/Brefeldin A; 20762-30-5/Adenosine Diphosphate Ribose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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