Document Detail

Existence of GPR40 functioning in a human breast cancer cell line, MCF-7.
MedLine Citation:
PMID:  14741707     Owner:  NLM     Status:  MEDLINE    
GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca(2+) concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells.
T Yonezawa; K Katoh; Y Obara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  314     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-26     Completed Date:  2004-03-17     Revised Date:  2006-05-26    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  805-9     Citation Subset:  IM    
Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, 981-8555, Sendai, Japan.
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MeSH Terms
Breast Neoplasms / metabolism*
Calcium / analysis,  metabolism
Cell Division / drug effects,  physiology
Cell Line, Tumor
Cytosol / metabolism
Dose-Response Relationship, Drug
Fatty Acids / antagonists & inhibitors,  chemistry,  pharmacology
Microscopy, Confocal
Pertussis Toxin / pharmacology
RNA, Messenger / biosynthesis,  genetics
Receptors, G-Protein-Coupled / biosynthesis,  genetics,  metabolism*
Signal Transduction
Reg. No./Substance:
0/FFAR1 protein, human; 0/Fatty Acids; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; 7440-70-2/Calcium; EC Toxin

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