| Existence of GPR40 functioning in a human breast cancer cell line, MCF-7. | |
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MedLine Citation:
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PMID: 14741707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca(2+) concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells. |
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Authors:
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T Yonezawa; K Katoh; Y Obara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 314 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2004-01-26 Completed Date: 2004-03-17 Revised Date: 2006-05-26 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 805-9 Citation Subset: IM |
Affiliation:
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Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, 981-8555, Sendai, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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metabolism* Calcium / analysis, metabolism Cell Division / drug effects, physiology Cell Line, Tumor Cytosol / metabolism Dose-Response Relationship, Drug Fatty Acids / antagonists & inhibitors, chemistry, pharmacology Humans Microscopy, Confocal Pertussis Toxin / pharmacology RNA, Messenger / biosynthesis, genetics Receptors, G-Protein-Coupled / biosynthesis, genetics, metabolism* Signal Transduction |
| Chemical | |
Reg. No./Substance:
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0/FFAR1 protein, human; 0/Fatty Acids; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; 7440-70-2/Calcium; EC 2.4.2.31/Pertussis Toxin |
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