Document Detail

Exhaled breath and fecal volatile organic biomarkers of chronic kidney disease.
MedLine Citation:
PMID:  23274524     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: While much is known about the effect of chronic kidney disease (CKD) on composition of body fluids little is known regarding its impact on the gases found in exhaled breath or produced by intestinal microbiome. We have recently shown significant changes in the composition of intestinal microbiome in humans and animals with CKD. This study tested the hypothesis that uremia-induced changes in cellular metabolism and intestinal microbiome may modify the volatile organic metabolites found in the exhaled breath or generated by intestinal flora.
METHODS: SD rats were randomized to CKD (5/6 nephrectomy) or control (sham operation) groups. Exhaled breath was collected by enclosing each animal in a glass chamber flushed with clean air, then sealed for 45 min and the trapped air collected. Feces were collected, dissolved in pure water, incubated at 37 degrees C in glass reactors for 24 h and the trapped air collected. Collected gases were analyzed by gas chromatography.
RESULTS: Over 50 gases were detected in the exhaled breath and 36 in cultured feces. Four gases in exhaled breath and 4 generated by cultured feces were significantly different in the two groups. The exhaled breath in CKD rats showed an early rise in isoprene and a late fall in linear aldehydes. The CKD animals' cultured feces released larger amounts of dimethyldisulfide, dimethyltrisulfide, and two thioesters.
CONCLUSIONS: CKD significantly changes the composition of exhaled breath and gaseous products of intestinal flora.
GENERAL SIGNIFICANCE: Analysis of breath and bowel gases may provide useful biomarkers for detection and progression of CKD and its complications.
Simone Meinardi; Kyu-Bok Jin; Barbara Barletta; Donald R Blake; Nosratola D Vaziri
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1830     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-05-14     Completed Date:  2013-06-20     Revised Date:  2014-01-20    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2531-7     Citation Subset:  IM    
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MeSH Terms
Aldehydes / analysis,  metabolism
Biological Markers / analysis,  metabolism
Breath Tests
Butadienes / analysis,  metabolism
Chromatography, Gas
Disease Models, Animal
Disease Progression
Disulfides / analysis,  metabolism
Feces / chemistry
Gases / analysis*,  metabolism
Hemiterpenes / analysis,  metabolism
Pentanes / analysis,  metabolism
Renal Insufficiency, Chronic / metabolism*,  pathology
Sulfides / analysis,  metabolism
Volatile Organic Compounds / analysis*,  metabolism
Grant Support
Reg. No./Substance:
0/Aldehydes; 0/Biological Markers; 0/Butadienes; 0/Disulfides; 0/Gases; 0/Hemiterpenes; 0/Pentanes; 0/Sulfides; 0/Volatile Organic Compounds; 0A62964IBU/isoprene; 3E691T3NL1/dimethyl trisulfide; 3P8D642K5E/dimethyl disulfide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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