Document Detail


Exhaled 8-isoprostane in sarcoidosis: relation to superoxide anion production by bronchoalveolar lavage cells.
MedLine Citation:
PMID:  20521080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: This study was designed to examine the mutual relationship between 8-isoprostane in exhaled breath condensate (EBC) and superoxide anion generation by bronchoalveolar lavage fluid (BALF) cells in patients with sarcoidosis.
DESIGN: About 29 patients with sarcoidosis, 34 healthy never smokers (control group for EBC) and 15 healthy never smokers (control group for BAL) were examined. EBC was collected directly before bronchoscopy. 8-Isoprostane was measured by ELISA, and superoxide anion by colorimetry.
RESULTS: Exhaled breath condensate 8-isoprostane is increased in sarcoidosis (median, 25-75 percentile): 2.50; 2.50-3.90 versus 6.20; 2.50-16.95 pg/ml, p ≤ 0.05). Spontaneous superoxide anion release from BALF cells was significantly elevated only in patients with a high percentage of lymphocytes in BALF (6.42 ± 1.24 vs. 23.52 ± 4.30 nmol/10(6) cells, p ≤ 0.01). There were no correlations between 8-isoprostane and spontaneous or stimulated superoxide anion release.
CONCLUSIONS: We confirmed higher concentrations of EBC 8-isoprostane in sarcoidosis and higher spontaneous release of superoxide anion from BALF cells in patients with sarcoidosis. The increase of EBC 8-isoprostane is not directly related to superoxide anion released from BALF cells.
Authors:
Wojciech J Piotrowski; Zofia Kurmanowska; Adam Antczak; Jerzy Marczak; Maciej Ciebiada; Paweł Górski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-03
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  59     ISSN:  1420-908X     ISO Abbreviation:  Inflamm. Res.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-11     Completed Date:  2011-02-28     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1027-32     Citation Subset:  IM    
Affiliation:
Department of Pneumology and Allergy, Medical University of Lodz, Kopcinskiego Str 22, 90-153, Lodz, Poland. piotrow@toya.net.pl
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / metabolism
Breath Tests
Bronchoalveolar Lavage Fluid / cytology*
Dinoprost / analogs & derivatives*,  metabolism
Exhalation
Humans
Oxidative Stress
Sarcoidosis / metabolism*,  pathology
Superoxides / metabolism*
Vasoconstrictor Agents / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Vasoconstrictor Agents; 11062-77-4/Superoxides; 27415-26-5/8-epi-prostaglandin F2alpha; 551-11-1/Dinoprost
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