Document Detail


Exercise training increases insulin-stimulated glucose disposal and GLUT4 (SLC2A4) protein content in patients with type 2 diabetes.
MedLine Citation:
PMID:  17019595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS/HYPOTHESIS: Exercise enhances insulin-stimulated glucose transport in skeletal muscle through changes in signal transduction and gene expression. The aim of this study was to assess the impact of acute and short-term exercise training on whole-body insulin-mediated glucose disposal and signal transduction along the canonical insulin signalling cascade. METHODS: A euglycaemic-hyperinsulinaemic clamp, with vastus lateralis skeletal muscle biopsies, was performed at baseline and 16 h after an acute bout of exercise and short-term exercise training (7 days) in obese non-diabetic (n=7) and obese type 2 diabetic (n=8) subjects. RESULTS: Insulin-mediated glucose disposal was unchanged following acute exercise in both groups. Short-term exercise training increased insulin-mediated glucose disposal in obese type 2 diabetic (p<0.05), but not in obese non-diabetic subjects. Insulin activation of (1) IRS1, (2) IRS2, (3) phosphotyrosine-associated phosphatidylinositol-3 kinase activity and (4) the substrate of phosphorylated Akt, AS160, a functional Rab GTPase activating protein important for GLUT4 (now known as solute carrier family 2 [facilitated glucose transporter], member 4 [SLC2A4]) translocation, was unchanged after acute or chronic exercise in either group. GLUT4 protein content was increased in obese type 2 diabetic subjects (p<0.05), but not in obese non-diabetic subjects following chronic exercise. CONCLUSIONS/INTERPRETATION: Exercise training increased whole-body insulin-mediated glucose disposal in obese type 2 diabetic patients. These changes were independent of functional alterations in the insulin-signalling cascade and related to increased GLUT4 protein content.
Authors:
D J O'Gorman; H K R Karlsson; S McQuaid; O Yousif; Y Rahman; D Gasparro; S Glund; A V Chibalin; J R Zierath; J J Nolan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-26
Journal Detail:
Title:  Diabetologia     Volume:  49     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-01-11     Completed Date:  2007-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2983-92     Citation Subset:  IM    
Affiliation:
Metabolic Research Unit, St James's Hospital, Trinity College, Dublin, Ireland.
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / blood
Biopsy
Blood Glucose / metabolism*
Blood Pressure
Diabetes Mellitus, Type 2 / blood,  complications,  metabolism*
Diabetic Diet
Exercise / physiology*
Exercise Test
Female
Glucose Clamp Technique
Glucose Transporter Type 4 / metabolism*
Humans
Insulin / blood,  physiology
Male
Middle Aged
Muscle, Skeletal / metabolism,  pathology
Obesity / blood,  complications,  metabolism,  physiopathology
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Glucose Transporter Type 4; 11061-68-0/Insulin; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase

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