Document Detail

Exercise training increases hepatic endoplasmic reticulum (er) stress protein expression in MTP-inhibited high-fat fed rats.
MedLine Citation:
PMID:  20373467     Owner:  NLM     Status:  MEDLINE    
The purpose of the study was: (1) to determine the effects of microsomal triglyceride transfer protein (MTP) inhibition on endoplasmic reticulum (ER) stress in liver, and (2) to determine if this response is altered in exercise-trained rats. Female Sprague-Dawley rats (6 weeks) fed either a standard (SD) or a high-saturated fat (HF; 43% as energy) diet were trained (Tr) or kept sedentary (Sed) for 6 week. Exercise training consisted of continuous running on a motor-driven rodent treadmill 5 times/week. Ten days before the end of these interventions, rats were administrated (ip) daily a MTP inhibitor (MTPX) or a placebo (P). MTPX injection resulted in a large (p < 0.01) liver triacylglycerol (TAG) accumulation in SD and HF-fed rats (approximately 200 mg g(-1)), irrespective of the training status, while plasma TAG levels were largely (approximately 80%) decreased (p < 0.01). MTPX injection in HF but not in SD-fed animals resulted in an increase in BiP/GRP78, ATF6, PERK, and XBP-1 mRNA levels, (p < 0.01) indicating an increase in the unfolding protein response (UPR) to ER stress. Interestingly, exercise training in rats fed the HF diet resulted in a further increase in BiP/GRP78 and XBP-1 mRNA levels in MTPX animals (p < 0.01). It is concluded that: (1) ER stress induced by MTPX occurs only in HF-fed rats despite the fact that liver TAG levels were largely increased in both dietary models; (2) the increase in gene expression of UPR markers with training may constitute a protective mechanism against ER stress in liver.
Natalie Ann Chapados; Jean-Marc Lavoie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  28     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-07     Completed Date:  2010-06-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  202-10     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 John Wiley & Sons, Ltd.
Department of Kinesiology, University of Montreal, Montr?al, Qu?bec, Canada.
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MeSH Terms
Biological Markers / metabolism
Body Composition
Carrier Proteins / antagonists & inhibitors*
Dietary Fats*
Endoplasmic Reticulum / metabolism*
Energy Metabolism
Gene Expression
Heat-Shock Proteins / genetics,  metabolism*
Liver / cytology,  metabolism*
Physical Conditioning, Animal / physiology*
Rats, Sprague-Dawley
Triglycerides / blood
Unfolded Protein Response
Reg. No./Substance:
0/Biological Markers; 0/Carrier Proteins; 0/Dietary Fats; 0/Heat-Shock Proteins; 0/Placebos; 0/Triglycerides; 0/microsomal triglyceride transfer protein

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