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Exercise training improves basal blood glucose metabolism with no changes of cytosolic IKK, JNK activation in skeletal muscle of OLETF rat.
MedLine Citation:
PMID:  21527545     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Redox-sensitive stress kinases and heat shock protein 72 (HSP72) have been considered to be associated with the development of type 2 diabetes in skeletal muscle. However, the effect of exercise training in skeletal muscle of type 2 diabetic models is largely unknown. The purpose of this study was to investigate the effect of 12-week exercise training, in gastrocnemius of type 2 diabetic rats, on the activation of c-Jun N-terminal Kinase (JNK), nuclear factor kappa B (NF-κB) pathway, and HSP72. Total hydroperoxide and 4-hydroxynoneal (4-HNE), as oxidative stress markers, were also examined. The Otsuka Long-Evans Tokushima fatty (OLEFT) rats were randomly divided into an exercise training group (Ex-OLETF, n=8) and a sedentary group (Sed-OLETF, n=8), while the Long-Evans Tokushima Otsuka (LETO) rats were used as a control group (Con-LETO, n=5). The Ex-OLETF rats were trained on a treadmill 5 times a week for 12 weeks. The levels of hydroperoxide and 4-HNE in both Ex-OLETF and Sed-OLETF were significantly higher compared with Con-LETO, but there was no difference between Ex-OLETF and Sed-OLETF. IκB kinase (IKK), JNK activation, and p65 nuclear translocation followed a similar pattern observed in oxidative stress markers. The level of HSP72 in Ex-OLETF was increased by exercise training, but it did not reach the level observed in Con-LETO. NF-κB DNA binding activity in Sed-OLETF was significantly higher compared with Con-LETO. Although it was not statistically significant, exercise training in Ex-OLETF showed the trend of reducing the activation of NF-κB DNA binding activity compared with Sed-OLETF (p=.104). Our findings indicate that exercise training improves basal glucose metabolism without the change of stress kinases, and that nuclear regulation of NF-κB activity in diabetic muscle could be regulated independent of the cytosolic pathway. Our study also suggests a possibility that exercise-induced HSP72 serves as a protective mechanism in skeletal muscle of OLETF rats.
Authors:
Hojun Lee; Hyukki Chang; Jae-Young Park; Si-Young Kim; Kyung-Mook Choi; Wook Song
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-28
Journal Detail:
Title:  Experimental physiology     Volume:  -     ISSN:  1469-445X     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Institute of Sports Science, Health & Exercise Science Lab, Seoul National University, Seoul, Korea;
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