| Exercise training has beneficial anti-atrophy effects by inhibiting oxidative stress-induced MuRF1 upregulation in rats with diabetes. | |
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MedLine Citation:
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PMID: 21620866 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: MuRF1 E3 ubiquitin ligase has been identified as a mediator of skeletal muscle wasting in various skeletal muscle atrophy models, and its expression is upregulated by oxidative stress. Exercise training could decrease oxidative stress and restore the atrophied skeletal muscle. Here, our aim was to investigate whether exercise training has any effect on MuRF1 expression in rats with diabetes. MAIN METHODS: Rats with streptozotocin-induced diabetes were subjected to exercise training, after which oxidative stress was determined, and MuRF1 expression was analyzed by immunohistochemistry, real-time RT-PCR and Western blot analysis. In addition, we analyzed C2C12 myotubes in an in vitro model to examine the effects of oxidative stress on the protein levels of MuRF1 and myosin heavy chain (MHC). KEY FINDINGS: While oxidative stress and MuRF1 expression were increased in rats with diabetes, exercise training diminished the skeletal muscle wasting in diabetic rats by decreasing oxidative stress and inhibiting MuRF1 expression at both the mRNA and protein levels. In addition, oxidative stress-induced MuRF1 upregulation promoted proteasome dependent degradation of the myosin heavy chain (MHC) in C2C12 myotubes. SIGNIFICANCE: Our study provides the first evidence that the beneficial anti-atrophy effects of exercise training on diabetes might be mediated by inhibiting oxidative stress-induced MuRF1 upregulation and preventing MuRF1-mediated degradation of MHC. |
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Authors:
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Guo-Qing Chen; Cai-Ying Mou; Yue-Qin Yang; Song Wang; Zhi-Wei Zhao |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2011-05-18 |
Journal Detail:
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Title: Life sciences Volume: 89 ISSN: 1879-0631 ISO Abbreviation: Life Sci. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-06-21 Completed Date: 2011-09-06 Revised Date: 2012-03-06 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 44-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Health Science, Wuhan Institute of Physical Education, Luoyu Road No. 461, Wuhan City, Hubei Province, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Diabetes Mellitus, Experimental / metabolism* Immunohistochemistry Muscle Fibers, Skeletal / metabolism Muscle Proteins / metabolism* Muscular Atrophy / prevention & control* Myosin Heavy Chains / metabolism Oxidative Stress / physiology* Physical Conditioning, Animal / physiology* Rats Reverse Transcriptase Polymerase Chain Reaction Ubiquitin-Protein Ligases / metabolism* Up-Regulation / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Muscle Proteins; 0/Myosin Heavy Chains; EC 6.3.2.19/Trim63 protein, rat; EC 6.3.2.19/Ubiquitin-Protein Ligases |
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